Nitric oxide-scavenging system for culturing oocytes, embryos, or other cells
AssigneeBoard of Supervisors of Louisiana State University and Agricultural and Mechanical College
InventorsWilliam Hansel, Jeong-Mook Lim
Life of this patent
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Abstract
Nitric oxide adversely affects survival and development of cells such as oocytes and embryos in vitro, particularly in a co-culture system. The addition of a nitric oxide inhibitor such as hemoglobin to such systems eliminates this toxic effect, and promotes mammalian oocytes, embryos, or other cells in vitro.
Claims18
A mammalian cell culture medium comprising nutrients conducive to the growth of mammalian cells in vitro, and an effective amount of a nitric oxide inhibitor.
A method for promoting the growth and development of a mammalian cell or cells, comprising growing the cell or cells in a culture medium comprising nutrients conducive to the growth of mammalian cells in vitro, and an effective amount of a nitric oxide inhibitor.
Dependencies are inferred from the claim text.