Protein purification I
AssigneeBiovitrum AB
InventorStefan Winge
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Abstract
PROTEIN PURIFICATION I The invention relates to processes for preparation of substantially pure antithrombin-III (AT-III), the antithrombin isoforms AT-IIIα and AT-IIIβ; and/or histidine-rich glycoprotein (HRGP). The processes comprise separating the said proteins on a cation exchange gel wherein the cation exchanger group is attached to the gel matrix via a linear polymer chain.
Claims23
A process for the preparation of a solution comprising substantially pure antithrombin-III (AT-III), or an isoform thereof, said process comprising the use of a cation exchange gel wherein the cation exchanger groups are attached to the gel matrix via a linear polymer chain.
A process for the preparation of a solution comprising a purified isoform of AT-Ill, comprising the steps
- (i) preparing a solution comprising substantially pure AT-III;
- (ii) contacting the said protein solution with a cation exchange gel wherein the cation exchanger group is attached to the gel matrix via a linear polymer chain, under conditions where AT-IIIα can be eluted from the gel under low ionic strength, while AT-IIlβ is attaching to the gel under low ionic strength conditions;
- and
- (iii) eluting, under low ionic strength conditions, and collecting a protein fraction, thereby obtaining a solution comprising substantially pure AT-IIIα;
- and/or
- (iv) eluting, under medium or high ionic strength conditions, and collecting a protein fraction, thereby obtaining a solution comprising AT-IIIβ.
Dependencies are inferred from the claim text.