Method of preparing a guanosine-group compound and an intermediate thereof
AssigneeYuki Gosei Kogyo Co., Ltd.
InventorsYoichi Mikami, Seiichiro Matsumoto, Yoshinori Hayashi, Toyoki Sato
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Abstract
A glyoxal-guanosine-group compound is prepared either by reacting glyoxal-guanine with any one of ribose-1-phosphate and 2-deoxyribose-1-phosphate in the presence of purine nucleoside phosphorylase, or by reacting glyoxal-guanine with any one selected from the group consisting of uridine, 2′-deoxyuridine and thymidine, together with phosphate ion, in the presence of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase. The glyoxal-guanosine-group compound is then decomposed by alkali, whereby a guanosine-group compound consisting of guanosine and 2′-deoxyguanosine is prepared.
Claims40
A method of preparing a glyoxal-guanosine-group compound represented by the formula (2):
- 15
- wherein R represents a hydrogen atom or a hydroxyl group, which comprises the step of;
- reacting glyoxal-guanine represented by the formula (1):
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- with ribose-1-phosphate or 2-deoxyribose-1-phosphate in the presence of purine nucleoside phosphorylase, thereby obtaining a glyoxal-guanosine or glyoxal-2′-deoxyguanosine.
A method of preparing a glyoxal-guanosine-group compound represented by the formula (2):
- 17
- wherein R represents a hydrogen atom or a hydroxyl group, which comprises the step of:
- reacting glyoxal-guanine represented by the formula (1):
- 18
- with any one selected from the group consisting of uridine, 2′-deoxyuridine and thymidine, together with phosphate ion, in the presence of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase, thereby obtaining a glyoxal-guanosine or glyoxal-2′-deoxyguanosine.
A method of preparing a guanosine-group compound, which comprises the steps of:
- reacting glyoxal-guanine represented by the formula (1):
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- with ribose-1-phosphate or 2-deoxyribose-1-phosphate in the presence of purine nucleoside phosphorylase, thereby obtaining a compound represented by the formula (2):
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- wherein R represents a hydrogen atom or a hydroxyl group;
- and
- decomposing, by alkali, the compound represented by the formula (2), thereby obtaining guanosine or 2′-deoxyguanosine.
A method of preparing a guanosine-group compound, which comprises the steps of:
- reacting glyoxal-guanine represented by the formula (1):
- 21
- with any one selected from the group consisting of uridine, 2′-deoxyuridine and thymidine, together with phosphate ion, in the presence of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase, thereby obtaining a compound represented by the formula (2):
- 22
- wherein R represents a hydrogen atom or a hydroxyl group;
- and
- decomposing, by alkali, the compound represented by the formula (2), thereby obtaining guanosine or 2′-deoxyguanosine.
Dependencies are inferred from the claim text.