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AssigneeGenencor International, Inc.
InventorsMerja E. Penttila, Michael Ward, Huaming Wang, Mari J. Valkonen, Markku L.A. Saloheimo
Estimating the term…
Described herein are methods for increasing the amount of protein secreted by a cell. In one case, a cell is provided which contains a heterologous nucleic acid encoding a protein having unfolded protein response modulating activity and a heterologous nucleic acid encoding a protein of interest to be secreted. In one case, the protein having unfolded protein response modulating activity is selected from the proteins selected from the group consisting of HAC1, PTC2 and lRE1. The protein of interest can be any secreted protein such as a therapeutic or an industrial enzyme. For example the protein can be selected from the group consisting of lipase, cellulase, endo-glucosidase H, protease, carbohydrase, reductase, oxidase, isomerase, transferase, kinase, phosphatase, alpha-amylase, glucoamylase, lignocellulose hemicellulase, pectinase and ligninase.
A method of increasing the secretion of a heterologous protein in a eukaryotic cell comprising inducing an elevated unfolded protein response (UPR).
An isolated nucleic acid encoding a HAC1 protein, wherein said HAC1 protein induces unfolded protein response and has less than 50% similarity to yeast HAC1 protein.
An isolated nucleic acid encoding a HAC1 protein, wherein said HAC1 protein induces unfolded protein response and wherein said HAC1 protein comprises a DNA binding region that has greater than 70% similarity to the DNA binding region of filamentous fungi HAC1 protein.
A protein having unfolded protein response inducing activity and having greater than 70% similarity to an amino acid sequence of FIG. 7 or FIG. 8 or FIG. 28.
A protein having an amino acid sequence as set forth in FIG. 7 or FIG. 8 or FIG. 28.
An isolated nucleic acid encoding a PTC2 protein wherein said PTC2 protein modulates unfolded protein response and wherein said PTC2 has at least 70% similarity to an amino acid sequence of FIG. 24 or FIG. 25.
A protein having unfolded protein response modulating activity and having greater than 70% similarity to the amino acid sequence of FIG. 24 or FIG. 25.
A protein having an amino acid sequence as set forth in FIG. 24 or FIG. 25.
A nucleic acid encoding a lRE1 protein having unfolded protein response modulating activity and having at least 60% to an amino acid sequence as shown in FIG. 26 or FIG. 27.
A protein having unfolded protein response inducing activity and having greater than 70% similarity to the amino acid sequence of FIG. 26 or FIG. 27.
A protein having an amino acid sequence as set forth in FIG. 26 or FIG. 27.
A cell containing a heterologous nucleic acid encoding a protein having unfolded protein response modulating activity and a heterologous nucleic acid encoding a protein of interest to be secreted.
Dependencies are inferred from the claim text.