Flavobacterium heparinum expression system
AssigneeBioMarin Pharmaceuticals
InventorsHongsheng Su, Zhongqi Shao, Ana Lydia Tkalec, Francoise Blain, Joseph Zimmerman
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Abstract
This invention is directed to Flavobacterium heparinum for use as a host cell organism for the expression of homologous and heterologous genes.
Claims29
A host cell for recombinant DNA expression comprising Flavobacterium heparinum.
A Flavobacterium heparinum host organism transformed with recombinant DNA comprising a homologous or a heterologous gene placed under the control of a gene promoter derived from a protein endogenous to the F. heparinum host and operably linked to the coding sequence for the homologous or heterologous gene.
A method for producing a desired polypeptide or protein comprising expressing recombinant DNA comprising a coding sequence for the desired polypeptide or protein in a F. heparinum host organism;
An expression system for expressing a desired polypeptide or protein comprising:
- (1) a F. heparinum host organism
- (2) nucleotide sequences encoding a desired polypeptide or protein, and
- (3) a vector for expressing the nucleotide sequences capable of expressing the desired polypeptide or protein.
A vector which upon introduction into a F. heparinum host cell effects expression of DNA encoding a desired polypeptide or protein, the vector comprising (a) a functional origin of replication (OriC) region;
- (b) replication (rep) genes;
- and (c) a gene promoter derived from a protein endogenous to the F. heparinum host.
The vector depicted in FIG. 1.
The vector depicted in FIG. 2.
Dependencies are inferred from the claim text.