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OwnerChiaki Saitoh, Hideyo Kumazawa +6inventors
InventorsChiaki Saitoh, Hideyo Kumazawa, Kazuo Aisaka, Toru Mizukami, Katsuhiko Ando, Keiko Ochiai, Ryoichi Katsumata, Kozo Ouchi
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According to the present invention, a process for producing a practical cholesterol-reduced substance by converting cholesterol contained in foods and feeds to coprostanol having very low intestinal tract absorbability by utilizing enzymatic action is provided. Cholesterol in a cholesterol-containing substance such as meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry and pisciculture, and the like, can be treated with three kinds of enzymes which are a cholesterol dehydrogenase having an optimum pH in a neutral pH range and 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase each having an optimum pH in a weak acidic range, or microbial cells containing the enzymes, for converting the cholesterol to coprostanol to reduce the amount of the cholesterol.
A process for producing a cholesterol-reduced substance, comprising converting cholesterol in a cholesterol-containing substance into coprostanol via cholesten-3-one and coprostan-3-one by contacting said cholesterol-containing substance with cholesterol dehydrogenase, 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase, wherein enzymatic action of said cholesterol dehydrogenase, 4-cholesten-3-dehydrogenase and coprostan-3-one dehydrogenase reduce amount of the cholesterol, and wherein the cholesterol dehydrogenase is a cholesterol dehydrogenase having a relative active value at pH=7.0 which is not less than 80% of the maximum active value.
A method for reducing the amount of cholesterol in a cholesterol-containing substance, by enzymatic action of cholesterol dehydrogenase, 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase on the cholesterol-containing substance, comprising contacting the cholesterol-containing substance with the cholesterol dehydrogenase, the 4-cholesten-3-one dehydrogenase and the coprostan-3-one dehydrogenase to reduce the amount of cholesterol in the cholesterol-containing substance by converting the cholesterol to coprostanol via 4-cholesten-3-one and coprostan-3-one, wherein the cholesterol dehydrogenase is a cholesterol dehydrogenase having a relative active value at pH=7 which is not less than 80% of the maximum active value.
Dependencies are inferred from the claim text.