Process for the isolation and/or purification of a proteinaceous material
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsHolger Rauth, Richard Reinhardt, Eckhard Nordhoff, Markus Kalkum
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Abstract
The invention relates to a process for the isolation and/or purification of a proteinaceous material, wherein a solid phase comprising a mixture of hydrophobic and hydrophilic groups is used.
Claims15
A process for the isolation and/or purification of a proteinaceous material comprising the steps:
- (a) providing an aqueous sample comprising a proteinaceous material,
- (b) contacting the aqueous sample with a solid phase comprising a mixture of hydrophobic groups and hydrophilic groups on at least one surface thereof, wherein said proteinaceous material binds to said at least one surface, and
- (c) separating off other sample components.
The process according to any of the preceding claims, wherein the molar ratio of hydrophobic to hydrophilic groups is from 10:1 to 1:10.
The process according to any of the preceding claims, wherein step (c) is performed by magnetic means.
The process according to any of the preceding claims, wherein the solid phase having proteinaceous material bound thereto is subjected to at least one washing step.
The process according to any of the preceding claims, further comprising the step
- (d) eluting the proteinaceous material from the solid phase.
The process according to any of the preceding claims, wherein the isolated and/or purified proteinaceous material is analyzed by mass spectrometry.
The process according to any of the preceding claims, wherein at least one process step is automated.
Dependencies are inferred from the claim text.