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US 2001/0020010 A1Published application
Histidine kinase
OwnerSanjoy Biswas, John P. Throup +2inventors
InventorsSanjoy Biswas, John P. Throup, Nicola G. Wallis, Magdalena Zalacain
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Abstract
The invention provides Histidine kinase polypeptides and polynucleotides encoding Histidine kinase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing Histidine kinase polypeptides to screen for antibacterial compounds.
Claims17
An isolated polypeptide selected from the group consisting of:
- (i) an isolated polypeptide comprising an amino acid having at least:
- (a) 70% identity;
- (b) 80% identity;
- (c) 90% identity;
- or
- (d) 95% identity to the amino acid sequence of SEQ ID NO:
- 2 or 4 over the entire length of SEQ ID NO:
- 2 or 4;
- (ii) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
- 2 or 4,
- (iii) an isolated polypeptide which is the amino acid sequence of SEQ ID NO:
- 2 or 4, and
- (iv) a polypeptide which is encoded by a recombinant polynucleotide comprising the polyncleotide sequence of SEQ ID NO:
- 2 or 3.
11 more elements
An isolated polynucleotide selected from the group consisting of:
- (i) an isolated polynucleotide comprising a polynucleotide sequence encoding a polypeptide that has at least
- (a) 70% identity;
- (b) 80% identity;
- (c) 90% identity;
- or
- (d) 95% identity;
- to the amino acid sequence of SEQ ID NO:
- 2 or 4, over the entire length of SEQ ID NO:
- 2 or 4;
- (ii) an isolated polynucleotide comprising a polynucleotide sequence that has at least:
- (a) 70% identity
- (b) 80% identity;
- (c) 90% identity;
- or
- (d) 95% identity;
- over its entire length to a polynucleotide sequence encoding the polypeptide of SEQ ID NO:
- 2 or 4;
- (iii) an isolated polynucleotide comprising a nucleotide sequence which has at least:
- (a) 70% identity;
- (b) 80% identity;
- (c) 90% identity;
- or
- (d) 95% identity;
- to that of SEQ ID NO:
- 1 or 3 over the entire length of SEQ ID NO:
- 1 or 3;
- (iv) an isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO:
- 2 or 4;
- (vi) an isolated polynucleotide which is the polynucleotide of SEQ ID NO:
- 1 or 3;
- (vi) an isolated polynucleotide obtainable by screening an appropriate library under stringent hybridization conditions with a probe having the sequence of SEQ ID NO:
- 1 or 3 or a fragment thereof;
- (vii) an isolated polynucleotide encoding a mature polypeptide expressed by the Histidine kinase gene contained in Streptococcus pneumoniae;
- and
- (viii) a polynucleotide sequence complementary to said isolated polynucleotide of (i), (ii), (iii), (iv), (v), (vi) or (vii).
32 more elements
A computer readable medium having stored thereon a member selected from the group consisting of:
- a polynucleotide comprising the sequence of SEQ ID NO. 1 or 3;
- a polypeptide comprising the sequence of SEQ ID NO. 2 or 4;
- a set of polynucleotide sequences wherein at least one of said sequences comprises the sequence of SEQ ID NO. 1 or 3;
- a set of polypeptide sequences wherein at least one of said sequences comprises the sequence of SEQ ID NO. 2 or 4;
- a data set representing a polynucleotide sequence comprising the sequence of SEQ ID NO. 1 or 3;
- a data set representing a polynucleotide sequence encoding a polypeptide sequence comprising the sequence of SEQ ID NO. 2 or 4;
- a polynucleotide comprising the sequence of SEQ ID NO. 1 or 3;
- a polypeptide comprising the sequence of SEQ ID NO. 2 or 4;
- a set of polynucleotide sequences wherein at least one of said sequences comprises the sequence of SEQ ID NO. 1 or 3;
- a set of polypeptide sequences wherein at least one of said sequences comprises the sequence of SEQ ID NO. 2 or 4;
- a data set representing a polynucleotide sequence comprising the sequence of SEQ ID NO. 1 or 3;
- a data set representing a polynucleotide sequence encoding a polypeptide sequence comprising the sequence of SEQ ID NO. 2 or 4.
9 more elements
A computer based method for performing homology identification, said method comprising the steps of providing a polynucleotide sequence comprising the sequence of SEQ ID NO. 1 or 3 in a computer readable medium;
- and comparing said polynucleotide sequence to at least one polynucleotide or polypeptide sequence to identify homology.
A further embodiment of the invention provides a computer based method for polynucleotide assembly, said method comprising the steps of:
- providing a first polynucleotide sequence comprising the sequence of SEQ ID NO. 1 or 3 in a computer readable medium;
- and screening for at least one overlapping region between said first polynucleotide sequence and a second polynucleotide sequence.
An isolated polynucleotide selected form the group consisting of:
- (a) an isolated polynucleotide comprising a nucleotide sequence which has at least 70%, 80%, 90%, 95%, 97% identity to SEQ ID NO:
- 3 over the entire length of SEQ ID NO:
- 3;
- (b) an isolated polynucleotide comprising the polynucleotide of SEQ ID NO:
- 3;
- (c) the polynucleotide of SEQ ID NO:
- 3;
- or
- (d) an isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide which has at least 70%, 80%, 90%, 95%, 97-99% identity to the amino acid sequence of SEQ ID NO:
- 4, over the entire length of SEQ ID NO:
- 4.
8 more elements
A polypeptide selected from the group consisting of:
- (a) a polypeptide which comprises an amino acid sequence which has at least 70%, 80%, 90%, 95%, 97-99% identity to that of SEQ ID NO:
- 4 over the entire length of SEQ ID NO:
- 4;
- (b) a polypeptide which has an amino acid sequence which is at least 70%, 80%, 90%, 95%, 97-99% identity to the amino acid sequence of SEQ ID NO:
- 4 over the entire length of SEQ ID NO:
- 4;
- (c) a polypeptide which comprises the amino acid of SEQ ID NO:
- 4;
- (d) a polypeptide which is the polypeptide of SEQ ID NO:
- 4;
- (e) a polypeptide which is encoded by a polynucleotide comprising the sequence contained in SEQ ID NO:
- 3.
9 more elements
Dependencies are inferred from the claim text.