Active retinoic acid-free culture medium for avian totipotent embryonic cells
OwnerJacques Samarut, Bertrand Paininventors
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Abstract
A culture medium for avian totipotential embryonic cells comprising an avian cell culture medium is disclosed. The culture medium is characterised in that it comprises elements complementary to said avian cell culture medium, the complementary elements being selected from the group which comprises cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors, and in that it is substantially free of active retinoic acid. A method for culturing avian totipotential embryonic cells, and the resulting products, are also disclosed.
Claims21
Culture medium for avian totipotent embryonic cells, of the type containing an avian cell culture medium, characterized in that it contains components supplementary to said avian cell culture medium, said supplementary components being chosen from the group comprising:
- cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors, and in that it is substantially free from active retinoic acid.
Method of culture of avian totipotent embryonic cells (or avian ES cells), characterized in that:
- a) cells originating from blastoderm disks of unfertilized [sic] eggs are suspended in an avian cell culture medium comprising, in addition, at least one compound chosen from cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors, and in which the retinoic acid is substantially inactivated,
- b) a lawn of feeder cells is inoculated with the suspension obtained after step a),
- c) the cells are incubated for a specified period,
- d) the cells in culture are removed and purified so as to recover bird ES cells.
Modified avian totipotent embryonic cell, characterized in that it can be obtained by integration of the gene coding for a heterologous protein in the genome of an avian ES cell in culture.
Dependencies are inferred from the claim text.