Recombinant microorganisms expressing antigenic proteins of helicobacter pylori

A chimeric protein which is prepared by ligating UreB of Helicobacter pylori and A2 and B subunits of Vibrio cholerae toxin, has an amino acid sequence represented as following, or its functional equivalents:

• MKISRKEYA SMYGPTTGDK VRLGDTDLIA EVEHDYTIYG EELKFGGGKT LREGMSQSNN PSKEELDLII TNALIVDYTG IYKADlGIKD GKIAGIGKGG NKDTQDGVKN NLSGPATEA LAGEGLIVTA GGIDTHIHFI SPQQIPTAFA SGVTTNIIGGG TGPADGTNAT TITPGRRNLK FMLRAAEEYS MNFGFLAKGN ASNDASLADQ IEAGAIGLKI HEDWGTTPSA INHALDVADK YDVQVAIHTD TLNEAGCVED TMAAIAGRTM HTYHTEGAGG GHAPDIIKVA GEHNILPAST NPTIPFTVNT EAEHMDMLMV CHHLDKSIKE DVQFADSRIR PQTIAAEDTL HDMGIFSITS SDSQAMGRVG EVITRTWQTA DKNKKEFGRL KEEKGDNDNF RIKRYLSKYT INPAIAHGIS EYVGSVEVGK VADLVLWSPA FFGVKPNMII KGGFIALSQM GDANASIPTP QPVYYREMFA HHGKAKYDAN ITFVSQAAYD KGIKEELGLE RQVLPVKNCR NITKKDMQFN DTTAHIEVNS ETYHVFVDGK EVTLNQPIKE FEEPWIHHAP PGCGNAPRSS ISNTCDEKTQ SLGVKFLDEY QSKVKRQIFS GYQSDIDTHN RIKDELMIKL KFGVFFTVLL SSAYAHGTPQ NITDLCAESH NTQIYTLNDK IFSYTESLAG KREMAIITFK NGAIFQVEVP SSQHIDSQKK AIERMKDTLR IAYLTEAKVE KLCVWNNKTP HAIAAISMAN.

A recombinant expression vector which is capable of expressing a chimeric protein consisting of UreB of Helicobacter pylori and A2 and B subunits of Vibrio cholerae toxin, represented as following genetic map:

• 1

A recombinant expression vector which is capable of expressing a chimeric protein consisting of CagA of Helicobacter pylori and A2 and 1B subunits of Vibrio cholerae toxin, represented as following genetic map:

• 2

Escherichia coli DW/HU-044(KCCM-10124) which is capable of expressing a chimeric protein consisting of UreB of Helicobacter pylori and A2 and B subunits of Vibrio cholerae toxin.

Escherichia coli DW/HC-033(KCCM-10123) which is capable of expressing a chimeric protein consisting of CagA of Helicobacter pylori and A2 and B subunits of Vibrio cholerae toxin.

A preventive and therapeutic vaccine for Helicobacter pylori-associated diseases which comprises an active ingredient of a chimeric protein consisting of antigenic protein of Helicobacter pylori and A2 and B subunits of Vibro cholerae toxin, and its pharmaceutically acceptable carrier.