Compositions, systems, and methods for creating in situ, chemically cross-linked, mechanical barriers

A biocompatible and biodegradable barrier material applied to seal a vascular puncture site comprising a mixture of a first liquid component and a second liquid component chemically cross-linked, without use of an enzyme, to form a non-liquid mechanical matrix.

A kit comprising

• a first dispenser containing a first liquid component,
• a second dispenser containing a second liquid component,
• instructions for handling the first and second dispensers according to a method comprising the steps of mixing the first and second liquid components to chemically cross-link the first and second components, without use of an enzyme, to form a non-liquid mechanical matrix, and applying the mechanical matrix to seal a vascular puncture site.

A method for sealing a vascular puncture site comprising the steps of

• creating, without use of an enzyme, a chemically cross-linked, non-liquid mechanical matrix, and
• locating the matrix to seal a vascular puncture site.

A method for creating a biocompatible and biodegradable barrier to seal a vascular puncture site comprising the steps of

• providing a first liquid component,
• providing a second liquid component, the first and second liquid components being free of an enzyme, and
• mixing the first and second liquid components by dispensing the components into a catheter tube deployed at the vascular puncture site, wherein, upon mixing, the first and second liquid components chemically cross-link to form a mechanical non-liquid matrix sealing the vascular puncture site.

A biocompatible barrier material comprising a mixture of a protein solution and a polymer solution including a derivative of a hydrophilic polymer with a functionality of at least three, wherein, upon mixing, the protein solution and the polymer solution cross-link to form a mechanical non-liquid matrix.

A method for creating a biocompatible barrier comprising the steps of mixing a protein solution and a polymer solution including a derivative of a hydrophilic polymer with a functionality of at least three, wherein, upon mixing, the protein solution and the polymer solution cross-link to form a mechanical non-liquid matrix.

A method for creating a biocompatible barrier comprising the steps of

• providing a protein solution,
• providing a polymer solution including a derivative of a hydrophilic polymer with a functionality of at least three, and
• mixing the protein and polymer solutions by dispensing the protein solution and the polymer solution into a channel, wherein, upon mixing, the protein solution and the polymer solution cross-link to form a mechanical non-liquid matrix.

A method for creating a biocompatible barrier to seal a vascular puncture site comprising the steps of

• providing a protein solution,
• providing a polymer solution including a derivative of a hydrophilic polymer with a functionality of at least three, and
• mixing the protein and polymer solutions by dispensing the protein solution and the polymer solution into a catheter tube deployed at a vascular puncture site, wherein, upon mixing, the protein solution and the polymer solution cross-link to form a mechanical non-liquid matrix sealing the puncture site.

A kit for forming a biocompatible barrier matrix comprising

• a first dispenser containing a protein solution,
• a second dispenser containing a polymer solution including a derivative of a hydrophilic polymer with a functionality of at least three, and
• instructions for handling the first and second dispensers to mix the protein solution and polymer solution, wherein, upon mixing, the protein solution and the polymer solution cross-link to form a mechanical non-liquid matrix.