Rh blood group antigen compositions and methods of use
AssigneeBoard of Regents
InventorsL. Scott Rodkey, Marwan A. Yared, Kenneth J. Moise JR.
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Abstract
Disclosed are novel protein and peptide compositions comprising soluble and bound forms of immunologically-active blood group antigens including mammalian Rh antigens. In preferred embodiments methods for the isolation and purification of serologically-active human Rh antigens such as D, c, C, E, and e are disclosed. Also disclosed are methods for the adsorption of immunologically-active Rh antigens to solid supports. Diagnostic kits, methods, and devices for the detection of Rh antibodies in clinical and non-clinical samples are also disclosed. Devices, compositions and methods for the isolation, purification and quantitation of anti-Rh antibodies from solution are also provided.
Claims77
A composition comprising an isolated and purified antigenically-active blood group antigen protein or peptide.
A method of stabilizing an antigenically-active blood group antigen protein or peptide, comprising admixing said antigenically-active blood group antigen protein or peptide with an effective amount of a low pH buffer.
An apparatus comprising a chamber having an inlet port and an outlet port, said chamber containing an immobilized antigenically-active blood group antigen.
A device comprising an antigenically-active blood group antigen protein or peptide.
A method of purifying an Rh antibody comprising:
- (a) contacting a sample suspected of containing said antibody with an immobilized antigen under conditions effective to bind said antibody;
- and
- (b) subsequently eluting said antibody from said immobilized antigen.
A method of removing an Rh antibody from a biological fluid comprising contacting said fluid with an immobilized Rh antigen under conditions effective to bind said antibody to said antigen.
Dependencies are inferred from the claim text.