Method of recovering a plurality of nucleic acids by an identical stationary phase and an apparatus thereof

A nucleic acid recovering method comprising

• a process of applying a plurality of specimens containing nucleic components to a stationary phase capable of binding with said nucleic components in a capillary to let said stationary phase bind with nucleic components in the specimen,
• a process of removing components which are left unabsorbed by said stationary phase,
• a process of applying a washing liquid into said capillary and discharging the washing liquid therefrom to wash the stationary phase,
• a process of repeating the above processes on each specimen to let the identical stationary phase absorb and bind with nucleic components, and
• a process of applying an eluting solution into said capillary, discharging said eluting solution therefrom, and eluting nucleic components from said stationary phase in said capillary.

A nucleic acid recovering method comprising

• a first process of preparing a plurality of specimens N (wherein “N” is the number of specimens) containing nucleic components,
• a second process of mixing at least part of said specimens and preparing specimens N/n (wherein “n” is an integer equal to or greater than 2 but smaller than “N” and “n” of respective mixed specimens can be identical or different) or a set of mixed specimens and single specimens,
• a third process of making said specimen (mixed or single) in contact with a stationary phase which can bind with nucleic components and letting said stationary phase absorb and bind with said nucleic acids in the specimen,
• a fourth process of removing the other components which are not absorbed and bound from said stationary phase,
• a fifth process of applying a washing liquid to said stationary phase, discharging said washing liquid, and thus washing said stationary phase,
• a sixth process of repeating said third to fifth processes on each of the other mixed and single specimens to absorb and bind nucleic acids to said stationary phase, and
• a seventh process of applying an eluting solution to said stationary phase, discharging the eluting solution therefrom, and thus eluting nucleic acids from said stationary phase.

A nucleic acid recovering method comprising

• a first process which comprises the steps of mixing a plurality of prepared specimens N (wherein “N” is the number of specimens) containing nucleic components, preparing mixed specimens N/n (wherein “n” is an integer equal to or greater than 3 but smaller than “N” and “n” of respective mixed specimens can be identical or different) or a set of mixed specimens and single specimens, making said specimen (mixed or single) in contact with a stationary phase which can bind with nucleic components of a specific structure in a container, and letting said stationary phase absorb and bind with said nucleic acids in the specimen,
• a second process which comprises a step of removing the other components which are not absorbed and bound from said stationary phase, and
• a third process which comprises the steps of applying a washing liquid to said stationary phase, discharging said washing liquid, and thus washing said stationary phase, and
• an additional process which comprises the steps of performing said first, second, and third processes on each of the mixed and single specimens to absorb and bind target nucleic components to an identical stationary phase, applying an eluting solution to said container and discharging from the container to elute nucleic components from the stationary phase in said container.

A nucleic acid recovering apparatus which applies specimens containing nucleic components in an area to a stationary phase capable of binding with said nucleic components to make said nucleic components be absorbed and bound by said stationary phase and elutes said nucleic components from said stationary phase for recovery, comprising

• means to scan said area in a predetermined sequence,
• first means to supply said specimen to said area,
• second means to remove components which are left unabsorbed from said stationary phase,
• third means to supply a washing liquid to said area, and
• means to apply an eluting solution to said stationary phase;
• wherein
• the apparatus controls to perform said first, second, and third means in sequence, to cause an identical stationary phase to absorb and bind with target nucleic components, and to apply an eluting solution to said stationary phase to elute nucleic components from said stationary phase.

A recording medium storing a processing program which comprises

• a step of applying specimens containing nucleic components to a stationary phase capable of binding with said nucleic components to make said nucleic components be absorbed by said stationary phase,
• a step of separating components which are left unabsorbed from said stationary phase,
• a step of performing said two steps on each of the other specimens to absorb said nucleic components to said stationary phase, and
• a step of applying an eluting solution to said stationary phase and discharging the solution from said stationary phase to elute said nucleic components from said stationary phase.