Method to obtain immortalized cell lines from primary cultured human keratinocytes
AssigneeProvincia Italiana Della Congragazione Dei Figli Dell' Immacolata Concezione-Instituto Dermopatico
InventorsMichele De Luca, Elena Dellambra
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Abstract
Immortalized cell lines from primary human keratinocytes cell lines obtained thanks to down regulation of 14-3-3-sigma coding gene. Said immortalized cell lines are extremely useful in in vivo and in vitro studies on cancer development. Infact, immortalization is absolutely required for somatic cells transformation. It is also an object of the present invention the immortalized cell lines obtained thereof.
Claims9
Method of immortalization of primary cell cultures of human keratinocytes, wherein said immortalization is obtained by down regulation and/or suppression of the expression of 14-3-3-sigma coding gene.
Cultured cell lines obtained from immortalized primary cell cultures of human keratinocytes by down regulating or inactivating 14-3-3-sigma expression.
Dependencies are inferred from the claim text.