Stimulation of hematopoietic cells in vitro
AssigneePoint Therapeutics, Inc.
InventorsWilliam Bachovchin, Barbara Wallner
Life of this patent
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Abstract
Methods, compositions, and devices for stimulating the number and/or differentiation of hematopoietic cells in vitro are provided. The methods involve contacting the hematopoietic cells with an inhibitor of dipeptidyl peptidase (DPIV) in the absence of exogenously provided cytokines.
Claims25
An apparatus comprising:
- (1) a container;
- and
- (2) an inhibitor of DPIV contained therein or attached thereto, wherein the container is sterile.
A composition comprising:
- (1) a magnetic particle;
- and
- (2) an inhibitor of DPIV attached thereto.
A kit for stimulating hematopoietic cells in vitro, comprising:
- (1) an apparatus comprising:
- (a) a container;
- and
- (b) an immobilized inhibitor of DPIV contained therein or attached thereto;
- and
- (2) instructions for using the apparatus to increase the number of hematopoietic cells in vitro.
A method for expanding antigen-specific T cells in vitro, comprising:
- (1) culturing bone marrow cells in the presence of a sufficient amount of a DPIV inhibitor to expand the number of early T lineage cells in the culture;
- and
- (2) culturing the early T lineage cells in the presence of a sufficient amount of a heteroconjugate containing an inhibitor of a DPIV inhibitor attached to an antigenic peptide to expand the number of antigen-specific T cells in the culture.
A method for expanding antigen-specific T cells in vitro, comprising:
- (1) culturing umbilical cord blood cells in the presence of a sufficient amount of a DPIV inhibitor to expand the number of early T lineage cells in the culture;
- and
- (2) culturing the early T lineage cells in the presence of a sufficient amount of a heteroconjugate containing an inhibitor of a DPIV inhibitor attached to an antigenic peptide to expand the number of antigen-specific T cells in the culture.
A method for expanding antigen-specific T cells in vitro, comprising:
- (1) culturing peripheral blood cells in the presence of a sufficient amount of a DPIV inhibitor to expand the number of T cells in the culture;
- and
- (2) culturing the T cells with a sufficient amount of a heteroconjugate containing an inhibitor of a DPIV inhibitor attached to an antigenic peptide to expand the number of antigen-specific T cells in the culture.
A method for expanding antigen-specific T cells in vitro, comprising:
- (1) culturing peripheral blood cells in the presence of a sufficient amount of a heteroconjugate containing an inhibitor of a DPIV inhibitor attached to an antigenic peptide to expand the number of antigen-specific T cells in the culture.
Dependencies are inferred from the claim text.