Alzheimer's disease secretase, APP substrates therefor, and uses therefor
AssigneePharmacia & Upjohn Company
InventorsMark E. Gurney, Michael J. Bienkowski, Robert L. Heinrikson, Luis A. Parodi, Riqiang Yan
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Abstract
The present invention provides the enzyme and enzymatic procedures for cleaving the β secretase cleavage site of the APP protein and associated nucleic acids, peptides, vectors, cells and cell isolates and assays. The invention further provides a modified APP protein and associated nucleic acids, peptides, vectors, cells, and cell isolates, and assays that are particularly useful for identifying candidate therapeutics for treatment or prevention of Alzheimer's disease.
Claims44
A purified polynucleotide comprising a nucleotide sequence encoding a polypeptide that comprises a fragment of a mammalian Asp2 protein, wherein said polypeptide lacks the Asp2 transmembrane domain of said Asp2 protein, and wherein the polypeptide and the fragment retain β-secretase activity of said Asp2 protein.
A polynulcleotide of claim 2 wherein the polypeptide comprises a fragment of human Asp2 protein.
A polynucleotide comprising a nucleotide sequence that hybridizes under stringent conditions to a nucleic acid comprising the sequence set forth in SEQ ID NO:
- 4 or SEQ ID NO:
- 6, wherein the nucleotide sequence encodes a polypeptide having β-secretase biological activity.
Dependencies are inferred from the claim text.