Process for preparing retrovirus vector for gene therapy

A DNA construction for regulating the expression of a foreign gene by using a recombinase and its recognition sequence wherein the LTR of a retrovirus genome and a packaging signal are followed by the recombinase recognition sequence, a drug resistance gene, a polyA addition signal, the recombinase recognition sequence, the foreign gene and LTR arranged in this order.

A short-lived transcript drug resistance gene characterized by having a base sequence of a short-lived mRNA of a neomycin resistance gene, a puromycin resistance gene or a hygromycin resistance gene.

A process for preparing a retrovirus vector for gene therapy which comprises transferring into retrovirus gag-pol-producing cells a DNA construction wherein a promoter, a recombinase recognition sequence, a drug resistance gene, a polyA addition signal, a recombinase recognition sequence, a virus structural protein gene and a polyA addition signal are arranged in this order and another DNA construction wherein the LTR of a retrovirus genome and a packaging signal are followed by a recombinase recognition sequence, a drug resistance gene, a polyA addition signal, a recombinase recognition sequence, a foreign gene and LTR arranged in this order, and then further transferring a DNA with the recombinase expression thereinto.

A process for preparing a retrovirus vector for gene therapy which comprises transferring into retrovirus gag-pol-env-producing cells a DNA construction wherein the LTR of a retrovirus genome and a packaging signal are followed by a recombinase recognition sequence, a drug resistance gene, a polyA addition signal, a recombinase recognition sequence, a foreign gene and LTR arranged in this order, and then further transferring a DNA with the recombinase expression thereinto.

A process for preparing a retrovirus vector for gene therapy which comprises transferring into retrovirus gag-pol-producing cells containing a retrovirus genome encoding a foreign gene a DNA construction wherein a promoter, a recombinase recognition sequence, a drug resistance gene, a polyA addition signal, a recombinase recognition sequence, a virus structural protein gene and a polyA addition signal are arranged in this order, and then further transferring a DNA with the recombinase expression thereinto.

A process for preparing a retrovirus vector whereby a pseudotyped retrovirus is prepared, characterized in that a negatively charged, high-molecular weight substance is contained in the liquid culture medium.