Electrophoretic method and electrophoretic instrument therefor
AssigneeHitachi, Ltd.
InventorsTomohiro Shoji, Yoshiyuki Okishima, Masaya Kojima, Muneo Maeshima
Life of this patent
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Abstract
An injection syringe 14 for injecting a gel into a capillary 111, a charging syringe 15 for charging the injection syringe 14 with the gel 10 are attached to a block 17. A check valve 16 is inserted between the both syringes, and the check valve 16 works so as to prevent the gel 10 from flowing back to the charging syringe 15. As the result, the gel charging can be automated, and accordingly the processing ability of the electrophoretic instrument can be improved.
Claims12
An electrophoresis method comprising the steps of supplying a gel for electrophoresis to a bore of an injection syringe;
- driving a plunger of said injection syringe by a motor;
- injecting the gel into a bore of a capillary through a block having a flow passage communicating to said capillary;
- and supplying an analyzed sample to said capillary to separate said sample by electrophoresis, the method further comprising the steps of:
- detecting an amount of the gel remaining in said injection syringe;
- driving a plunger of a charging syringe to charge said flow passage in said block with the gel when the value of amount is smaller than a predetermined amount of the gel, the charging syringe being connected to said flow passage in said block through a check valve;
- then
- supplying the gel to said capillary by the plunger of said injection syringe.
An electrophoretic instrument comprising:
- a capillary;
- a gel injection mechanism for injecting a gel of a sample separating medium into said capillary;
- a detecting unit for detecting a sample introduced into said capillary and electrophoretically separated;
- an injection syringe with a plunger for injecting said gel into said capillary;
- a charging syringe with a plunger for performing charging with said gel;
- a block in which a flow passage connecting among said syringes, said capillary and a buffer reservoir is formed;
- a check valve for preventing said gel from flowing back to said charging syringe, said check valve being arranged between said charging syringe and said flow passage;
- and
- an encoder for obtaining an amount of the gel in said injection syringe.
An electrophoretic instrument comprising:
- a capillary;
- a gel injection mechanism for injecting a gel of a sample separating medium into said capillary;
- a detecting unit for detecting a sample introduced into said capillary and electrophoretically separated;
- an injection syringe with a plunger for injecting said gel into said capillary;
- a charging syringe with a plunger for performing charging with said gel;
- a block in which a flow passage connecting among said syringes, said capillary and a buffer reservoir is formed;
- a check valve for preventing said gel from flowing back to said charging syringe, said check valve being arranged between said charging syringe and said flow passage;
- and
- an encoder for detecting positional information of a drive unit.
Dependencies are inferred from the claim text.