Microscope

An epi-fluorescence microscope comprising:

• a light guiding device that guides illuminating light emitted by a light source by reflecting the illuminating light to implement epi-lighting on a test piece, and guides fluorescent light excited by the illuminating light and generated from the test piece to an observation unit by allowing the fluorescent light to be transmitted;
• and
• a frame body that holds said light guiding device on an optical axis of an observation optical system, wherein
• an opening is provided at a wall surface of said frame body intersecting an optical path of transmitted light that is a part of the illuminating light and has been transmitted through said light guiding device instead of being reflected at said light guiding device.

An epi-fluorescence microscope comprising:

• a light guiding device that guides illuminating light emitted by a light source by reflecting the illuminating light to implement epi-lighting on a test piece, and guides fluorescent light excited by the illuminating light and generated from the test piece to an observation unit by allowing the fluorescent light to be transmitted;
• a frame body that hods said light guiding device on an optical axis of an observation optical system;
• a first light attenuating device that is provided on an optical path of transmitted light which is a part of the illuminating light and has been transmitted through said light guiding device instead of being reflected by said light guiding device, and attenuates a quantity of the transmitted light;
• and
• one or more second light attenuating devices that are provided on an optical path along which light having been attenuated at said first light attenuating device advances, and further attenuates a quantity of an attenuated transmitted light.

An epi-fluorescence microscope comprising:

• a plurality of light guiding devices each provided to guide illuminating light emitted by a light source by reflecting the illuminating light to implement epi-lighting on a test piece, and guide fluorescent light excited by the illuminating light and generated from the test piece to an observation unit by allowing the fluorescent light to be transmitted;
• a holding device that selectively holds one of said plurality of light guiding devices at an optical axis of an observation optical system;
• and
• a light attenuating device that prevents transmitted light, which is a part of the illuminating light entering said light guiding device held at the optical axis and has been transmitted through said held light guiding device instead of being reflected by said held light guiding device, from being reflected to be allowed to advance toward said light guiding device.

A microscope, comprising;

• a light guiding device that guides illuminating light emitted from a light source to illuminate a test piece and guides light from the test piece toward an observation unit;
• and
• a member that is provided at a position outside an observation optical path and having a surface inclined relative to an optical path of said light guiding device, wherein:
• said light guiding device guides the illuminating light to an objective lens group which irradiates the test piece and guides a part of the illuminating light to a position other than said objective lens group to result in generation of stray light;
• and
• said stray light is eliminated at a preceding stage before said stray light enters said objective lens group by said member having said inclined surface.