Chromatin associated proteins
OwnerRebecca E. Cahoon, Joan T. Odell +2inventors
InventorsREBECCA E. CAHOON, JOAN T. ODELL, GUO-HUA MIAO, HAJIME SAKAI
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a chromatin associated protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the chromatin associated protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the chromatin associated protein in a transformed host cell.
Claims11
An isolated nucleic acid fragment encoding a histone deacetylase 3 protein comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:
- 2, 4, 6 and 8;
- (b) an isolated nucleic acid fragment that is complementary to (a).
A histone deacetylase 3 polypeptide comprising the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:
- 2, 4, 6 and 8.
A method of altering the level of expression of a chromatin associated protein in a host cell comprising:
- (a) transforming a host cell with the chimeric gene comprising a nucleic acid fragment that encodes an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:
- 2, 4, 6 and 8;
- and
- (b) growing the transformed host cell produced in step (a) under conditions that are suitable for expression of the chimeric gene
- wherein expression of the chimeric gene results in production of altered levels of a chromatin associated protein in the transformed host cell.
A method of obtaining a nucleic acid fragment encoding a substantial portion of an amino acid sequence encoding a chromatin associated protein comprising:
- (a) synthesizing an oligonucleotide primer corresponding to a portion of the sequence set forth in any of SEQ ID NOs:
- 1, 3, 5 and 7;
- and
- (b) amplifying a cDNA insert present in a cloning vector using the oligonucleotide primer of step (a) and a primer representing sequences of the cloning vector
- wherein the amplified nucleic acid fragment encodes a substantial portion of an amino acid sequence encoding a chromatin associated protein.
Dependencies are inferred from the claim text.