# US 12,767,569 B2 · Resource-circulation-type and eco-friendly livestock manure treatment method using algal biomass, and system for producing algal biomass used therein > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,767,569 B2 - Document ID: US12767569B2 - Status: Granted - Kind: B2 - Application number: 18/551,130 - Filed: 2022-04-01 - Published: 2024-05-16 - Granted: 2026-10-20 - Earliest claimed: 2021-05-18 - Assignees: Korea Institute of Ocean Science & Technology - Applicants: Korea Institute of Ocean Science & Technology - Inventors: Do-Hyung Kang; Yong-Kyun Ryu; Won-Kyu Lee; Woon-Yong Choi; Tae-Ho Kim; Yeon-Ji Lee; Areumi Park; Youn Sik Jeong - Web page: https://patentgrep.com/patent/US12767569B2 ## CPC - C02F 3/322: {use of algae} - A01G 33/00: Cultivation of seaweed {or algae} - A23K 10/30: from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10) - C02F 3/32: characterised by the animals or plants used, e.g. algae - C02F 9/00: Multistage treatment of water, waste water or sewage - C05F 3/00: Fertilisers from human or animal excrements, e.g. manure - C12M 1/00: Apparatus for enzymology or microbiology - C12M 1/06: with agitator, e.g. impeller - C12M 1/34: Measuring or testing with condition measuring or sensing means, e.g. colony counters - C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00) - C12N 1/125: Unicellular algae isolates - C02F 2101/105: {Phosphorus compounds} - C02F 2101/16: Nitrogen compounds, e.g. ammonia - C02F 2103/20: from animal husbandry - C02F 2203/006: details of construction, e.g. specially adapted seals, modules, connections - C12R 2001/89: Algae {; Processes using algae} - Y02E 50/30: Fuel from waste, e.g. synthetic alcohol or diesel - Y02W 30/40: Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse - C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes) - A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12) - A23K: FODDER - C05F: ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS - Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION - Y02W: CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT ## Abstract The present disclosure provides a resource-circulation-type and eco-friendly livestock manure treatment method, and a system for producing algal biomass used therein, the method being capable of: preventing water pollution while allowing livestock liquid fertilizer to be treated on the basis of a biological process; producing algal biomass; and supplying the produced algal biomass to livestock feed and farms. ## Claims ### Claim 1 A resource-circulation-type and eco-friendly livestock manure treatment method using algal biomass, the method comprising: (a) an algae selection step of preparing algae capable of being cultured and proliferating as inoculum; (b) a clean culture step of culturing the selected algae in a culture medium in which a livestock liquid fertilizer is mixed with water to produce algal biomass, in an algal biomass production system; (c) a feed material production step of harvesting the algal biomass, followed by drying and pulverizing to obtain algal biomass powder; and (d) a post-treatment step of subjecting the culture medium remaining after harvesting the algal biomass in the clean culture step with water treatment, followed by spraying or discharging, wherein in step (b), the algae culture is performed by culturing microalgae, followed by culturing marine algae. ### Claim 2 (depends on 1) The method of claim 1, wherein the algae is at least one selected from the group consisting of microalgae and marine algae. ### Claim 3 (depends on 2) The method of claim 2, wherein the microalgae comprises at least one selected from the group consisting of Spirulina sp., Chlorella sp., Scenedesmus sp., Chlorococcum sp., Chlamydomonas sp., Microcystis sp. and Euglena sp. ### Claim 4 (depends on 3) An animal feed material containing algal biomass powder obtained by the resource-circulation-type and eco-friendly livestock manure treatment method using algal biomass according to claim 3. ### Claim 5 (depends on 2) The method of claim 2, wherein the marine algae comprises at least one selected from the group consisting of Enteromorpha sp., Ulva sp., Saccharina sp., Garcilaria sp. and Sargassum sp. ### Claim 6 (depends on 5) An animal feed material containing algal biomass powder obtained by the resource-circulation-type and eco-friendly livestock manure treatment method using algal biomass according to claim 5. ### Claim 7 (depends on 2) An animal feed material containing algal biomass powder obtained by the resource-circulation-type and eco-friendly livestock manure treatment method using algal biomass according to claim 2. ### Claim 8 (depends on 1) The method of claim 1, wherein in the culture medium of step (b), the liquid fertilizer and water are mixed in a volume ratio of 1:9 to 9:1. ### Claim 9 (depends on 8) An animal feed material containing algal biomass powder obtained by the resource-circulation-type and eco-friendly livestock manure treatment method using algal biomass according to claim 8. ### Claim 10 (depends on 1) The method of claim 1, wherein the harvesting of the algal biomass is performed by at least one of filtration, sedimentation, flotation, centrifugation and flocculation. ### Claim 11 (depends on 10) An animal feed material containing algal biomass powder obtained by the resource-circulation-type and eco-friendly livestock manure treatment method using algal biomass according to claim 10. ### Claim 12 (depends on 1) The method of claim 1, wherein the drying is performed by at least one of freeze drying, far-infrared ray drying, vacuum drying, hot air drying, spray drying, drum drying and natural drying. ### Claim 13 (depends on 12) An animal feed material containing algal biomass powder obtained by the resource-circulation-type and eco-friendly livestock manure treatment method using algal biomass according to claim 12. ### Claim 14 (depends on 1) The method of claim 1, wherein the water treatment is performed to meet effluent water quality standards of Biological Oxygen Demand (BOD, mg/L) of 30 or less; Chemical Oxygen Demand (COD, mg/L) of 50 or less; Total Organic Carbon (TOC, mg/L) of 3000 or less; Total Nitrogen (T-N, mg/L) of 60 or less; Total phosphorus (T-P, mg/L) of 8 or less; Suspended matter amount (SS, mg/L) of 30 or less; and Total Coliform Count (number/mL) of less than 3000. ### Claim 15 (depends on 1) The method of claim 1, wherein the method is performed using an algal biomass production system comprising: a controller for controlling culture environment; a medium preparation tank for preparing the culture medium for culturing the microalgae or the marine algae; a first culture tank for culturing the microalgae by introducing the culture medium and the microalgae as the inoculum into; a first filtration tank for filtering the microalgae and the culture medium cultured in the first culture tank; a second culture tank for culturing the marine algae by introducing the culture medium obtained from the first filtration tank and the marine algae as the inoculum into; and a second filtration tank for filtering the marine algae and the culture medium cultured in the second culture tank. ### Claim 16 (depends on 15) The method of claim 15, wherein the first culture tank and the second culture tank comprise at least one of a raceway pond and a photo-bioreactor. ### Claim 17 (depends on 15) The method of claim 15, wherein the first culture tank and the second culture tank comprise an agitator of at least one of a screw type, a paddle type, a propeller type and a water wheel type. ### Claim 18 (depends on 15) The method of claim 15, wherein the first culture tank and the second culture tank further comprise a heating device and a cooling device for maintaining an optimal culture temperature in the culture tank. ### Claim 19 (depends on 1) An animal feed material containing algal biomass powder obtained by the resource-circulation-type and eco-friendly livestock manure treatment method using algal biomass according to claim 1. ### Claim 20 (depends on 19) The animal feed material of claim 19, which is used as a raw material for feed additives or feed compositions.