# US 12,765,501 B2 · Alfalfa variety R0820SN520

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- Number: US 12,765,501 B2
- Document ID: US12765501B2
- Status: Granted
- Kind: B2
- Application number: 18/588,233
- Filed: 2024-02-27
- Published: 2025-08-28
- Granted: 2026-10-20
- Earliest claimed: 2024-02-27
- Assignees: Forage Genetics International, LLC
- Applicants: Forage Genetics International, LLC
- Inventors: Julie Ho; Fabio Pedraza
- Web page: https://patentgrep.com/patent/US12765501B2

## CPC

- A01H 6/544: {Medicago sativa [alfalfa]}
- A01H 5/10: Seeds
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The invention relates to the alfalfa variety designated R0820SN520. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R0820SN520. Also provided by the invention are tissue cultures of the alfalfa variety R0820SN520 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R0820SN520 with itself or another alfalfa variety and plants produced by such methods.

## Claims

### Claim 1

A population of seeds of alfalfa variety R0820SN520, wherein representative seed of said alfalfa variety have been deposited under NCMA Accession No. 202310008; and wherein a population of plants produced from the population of seeds has all of the physiological and morphological characteristics of said alfalfa variety R0820SN520.

### Claim 2 (depends on 1)

A population of plants grown from the population of seeds of claim 1.

### Claim 3 (depends on 1)

A composition comprising the population of seeds of claim 1 that is comprised in plant seed growth media.

### Claim 4 (depends on 3)

The composition of claim 3, wherein the plant seed growth media is soil or a synthetic cultivation media.

### Claim 5 (depends on 2)

A method for producing a population of first generation progeny alfalfa seeds, the method comprising crossing the population of plants of claim 2 with a second alfalfa plant population or inter-pollinating the population of plants of claim 2 and harvesting the resultant alfalfa seeds.

### Claim 6 (depends on 5)

The method of claim 5, wherein the second alfalfa plant population is a population of plants of alfalfa variety R0820SN520; wherein representative seed of said alfalfa variety have been deposited under NCMA Accession No. 202310008.

### Claim 7 (depends on 6)

A population of first generation progeny alfalfa seeds produced by the method of claim 6; wherein the population of plants produced from the first generation progeny alfalfa seeds has all of the physiological and morphological characteristics of said alfalfa variety R0820SN520.

### Claim 8 (depends on 7)

A population of alfalfa plants produced by growing the seeds of claim 7; wherein the population of plants produced has all of the physiological and morphological characteristics of said alfalfa variety R0820SN520.

### Claim 9 (depends on 2)

A method of vegetatively propagating the population of plants of claim 2, the method comprising the steps of:
(a) collecting tissue samples capable of being propagated from the population of plants of claim 2;
(b) cultivating the tissue to obtain proliferated shoots; and
(c) rooting the proliferated shoots to obtain rooted plantlets.

### Claim 10 (depends on 9)

The method of claim 9, further comprising growing a plant from the rooted plantlets.

### Claim 11 (depends on 2)

A method of modifying a population of alfalfa plants, wherein the method comprises introducing a transgene into the population of plants of claim 2, wherein the transgene is introduced by genetic transformation.

### Claim 12 (depends on 11)

The method of claim 11, wherein the transgene comprises a nucleic acid sequence that enables site-specific genetic recombination or confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, improved digestibility, improved energy content, improved forage yield, improved seed yield, improved winterhardiness, improved nitrogen fixation, modified fatty acid metabolism, abiotic stress resistance, flowering time, altered seed amino acid composition, and modified carbohydrate metabolism.

### Claim 13 (depends on 2)

A method for introducing a transgene into a population of alfalfa plants, the method comprising the steps of:
(a) modifying a plant of the population of plants of claim 2 by introducing a transgene; and
(b) crossing the modified alfalfa plant of step (a) with a population of alfalfa plants to produce a population of progeny plants, wherein at least a progeny plant comprises the transgene.

### Claim 14 (depends on 13)

The method of claim 13, further comprising the step of:
(c) applying a selection technique to the population produced in step (b) to select said progeny plants that comprise the transgene.

### Claim 15 (depends on 1)

A method of producing a synthetic alfalfa variety, the method comprising intercrossing the seeds of claim 1 with seeds of a second alfalfa variety.

### Claim 16 (depends on 2)

A method of producing a commodity plant product, the method comprising obtaining the population of plants of claim 2 and producing the commodity plant product from the population of plants, wherein the commodity plant product is selected from the group consisting of sprouts, forage, hay, greenchop, and silage.
