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US 12,761,231 B2Granted
Method for determining the level of vitamin D and metabolites thereof
AssigneeRoche Diagnostics Operations, Inc.
InventorsSilvia Baecher, Andrea Geistanger, Dominik Gruber, Hans-Peter Josel
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Abstract
The present invention relates to a method and the use thereof for determining the level of Vitamin D and metabolites thereof. Further, it is an object of the present invention to provide a kit and the use thereof for determining the level of Vitamin D and metabolites thereof.
Claims13
A method for determining the level of Vitamin D and metabolites thereof in a sample comprising:
- a) treating the sample with a releasing reagent, wherein the releasing reagent is provided in an effective level to release Vitamin D and metabolites thereof from a protein or a lipid present in the sample,
- wherein the method is free of the addition of sodium salicylate as the releasing reagent,
- wherein the releasing reagent is a salt comprising:
- a benzoate anion,
- one or two hydroxyl groups, which are linked to the phenyl group of the benzoate anion,
- optionally at least one residue having a molar mass of at least 15 g/mol, which is linked to the phenyl group of the benzoate anion, and
- a sodium cation or an ammonium cation, or
- wherein the releasing reagent is 3-hydroxybenzoic acid or 2,4-dihydroxybenzoic acid,
- b) optionally purification of the treated sample obtained from step a), and
- c) determining the level of the Vitamin D and metabolites thereof released in step a) using mass spectrometry.
7 more elements
Dependencies are inferred from the claim text.