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US 12,761,230 B2Granted
Method for diagnosing diseases
AssigneeNewomics Inc.
InventorsDaojing Wang, Pan Mao, Weimin Ni
Life of this patent
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Abstract
The present disclosure provides methods and systems for diagnosing diseases and monitoring their progression and therapeutic responses by detecting a presence or absence, or an increase or decrease, of one or more substances in a sample. The methods may involve microflow liquid chromatography (LC) coupled with mass spectrometry (MS) to rapidly quantitate biomarkers in a sample and identify the likelihood of the sample being positive for a disease or condition.
Claims20
A method for determining a likelihood that a subject has Alzheimer's Disease, comprising:
- (a) via immunoprecipitation, enriching a biological sample of said subject for proteins that are associated with said Alzheimer's Disease;
- (b) directing a liquid medium comprising at least a subset of said proteins enriched in (a) to flow through a microfluidic device to separate individual proteins of said at least said subset of said proteins from one another;
- (c) performing a targeted mass spectrometry (MS) on separated individual proteins obtained in (b) by selecting a preferred charge state of a subset of said individual proteins and generating signals assignable to said subset of individual proteins or fragments thereof;
- and
- (d) determining, based at least in part on said signals generated in (c), said likelihood that said subject is positive for said Alzheimer's Disease.
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Dependencies are inferred from the claim text.