A microfluidic chip for an in-situ electrical impedance spectroscopy (EIS) detection of Caenorhabditis elegans (C. elegans), comprising a glass substrate layer, a fluidic channel layer, and a pneumatic valve channel layer, wherein the glass substrate layer, the fluidic channel layer, and the pneumatic valve channel layer are connected by means of screws;
- the glass substrate layer, as a base of the microfluidic chip, is configured for supporting upper structures, and a micro-electrode array is integrated on the glass substrate layer;
- the fluidic channel layer comprises a C. elegans perfusion channel, a storage chamber, a deflection channel, an EIS measurement chamber, and a fluidic outlet channel;
- wherein the C. elegans perfusion channel, the storage chamber, the deflection channel, the EIS measurement chamber, and the fluidic outlet channel are connected in sequence;
- a measurement medium injection channel is connected between the deflection channel and the EIS measurement chamber;
- wherein the storage chamber is configured for storing C. elegans samples to be measured;
- wherein the deflection channel allows the C. elegans to deflect therein freely;
- wherein the EIS measurement chamber is in indirect contact with electrodes below;
- wherein the pneumatic valve channel layer comprises storage chamber control valves, a deflection channel control valve, a C. elegans capture valve, and a C. elegans immobilizing valve, wherein two of the storage chamber control valves are respectively located above slits in an inlet side and an outlet side of the storage chamber and configured for controlling the C. elegans to enter or come out of the storage chamber;
- wherein the deflection channel control valve is located above slits between the deflection channel and the EIS measurement chamber and configured for controlling the C. elegans to enter the EIS measurement chamber;
- wherein the C. elegans capture valve is located above slits between the EIS measurement chamber and the fluidic outlet channel and configured for controlling the C. elegans in the EIS measurement chamber to be captured and released;
- wherein the C. elegans immobilizing valve is located above the EIS measurement chamber, a pneumatic valve channel has a width greater than a width of the EIS measurement chamber, and the C. elegans immobilizing valve is configured for immobilizing the C. elegans entering a measurement channel;
- wherein the micro-electrode array comprises square electrode pins located around the microfluidic chip and connected to a measurement circuit, and functional structures located around the EIS measurement chamber and the deflection channel;
- wherein the functional structures are divided into two groups, a first group comprises deflection electrode pairs located on two sides of the deflection channel and configured for controlling the C. elegans to deflect, and a second group comprises EIS measurement electrodes located around the EIS measurement chamber and configured for measuring EIS signals of the C. elegans at different positions;
- wherein the storage chamber and the storage chamber control valves on the inlet side and the outlet side of the storage chamber form a C. elegans storage region;
- a one-time injection and a storage of the C. elegans is completed in the C. elegans storage region;
- when the storage chamber control valve on the inlet side of the storage chamber is opened and the storage chamber control valve on the outlet side of the storage chamber is closed, the C. elegans is injected;
- when the storage chamber control valve on the inlet side of the storage chamber is closed and the storage chamber control valve on the outlet side of the storage chamber is opened, the C. elegans enters the deflection channel;
- wherein the EIS measurement chamber, the measurement medium injection channel, the EIS measurement electrodes, the C. elegans capture valve, and the C. elegans immobilizing valve form an EIS measurement region-;
- an in-situ EIS measurement of the C. elegans on a plurality of sites is implemented in the EIS measurement region;
- wherein the deflection channel, the deflection electrode pairs, and the deflection channel control valve form a C. elegans deflection region;
- deflection of the C. elegans to enter the EIS measurement region is implemented in the C. elegans deflection region;
- wherein the C. elegans storage region, the C. elegans deflection region, and the EIS measurement region form a measurement line.