Predicting efficacy of or resistance to treatment of colon cancer
Assignee2cureX A/S
InventorsGrith Hagel, Jacob Thastrup, Ole Thastrup
Life of this patent
Estimating the term…
Abstract
The invention relates to methods for predicting the efficacy and/or resistance to one or more treatment regimens comprising administration of a platinum compound in individuals suffering from colon cancer. The methods comprises providing a colon cancer biopsy from the individual, generating tumoroids thereof, and testing whether growth/viability/metabolism of said tumorids is inhibited by incubation with the platinum compounds.
Claims20
A method for predicting the efficacy of treatment of colon cancer with one or more different treatments in an individual suffering from said cancer or for predicting resistance to treatment with one or more different treatments of colon cancer in an individual suffering from said cancer, wherein each treatment is treatment with a platinum compound, said method comprising the steps of
- a) providing at least one colon cancer biopsy obtained from said individual, wherein the biopsy comprises or consists of colon cancer tissue from the primary colon cancer, from lymph nodes or from one or more mesastases of said colon cancer,
- b) dissociating the biopsy into tissue fragments comprising a plurality of cells attached to each other,
- c) incubating said tissue fragments in a cell-compatible support, which supports 3 dimensional maintenance and/or growth of human or other mammalian cells to generate tumoroids, wherein the cell-compatible support is a sol-gel with a sol-gel transition temperature in the range of 0 to 35° C.,
- d) incubating a random selection of said tumoroids with the platinum compound of each treatment under conditions supporting 3 dimensional maintenance and/or growth of human or other mammalian cells,
- e) determining whether growth of said tumoroids is inhibited by incubation with said platinum compound,
- wherein inhibition of growth of said tumoroids by said platinum compound of a given treatment is indicative of efficacy of said treatment of colon cancer in said individual.
A method of identifying a platinum compound, which alone or in combination with one or more additional anti-cancer compounds are likely to be efficient in treatment of colon cancer in an individual in need thereof, said method comprising the steps of
- a) providing at least one colon cancer biopsy obtained from said individual, wherein the biopsy comprises or consists of colon cancer tissue from the primary colon cancer, from lymph nodes or from one or more metastases of said colon cancer,
- b) dissociating the biopsy into tissue fragments comprising a plurality of cells attached to each other,
- c) incubating said tissue fragments in a cell-compatible support, which supports 3 dimensional maintenance and/or growth of human or other mammalian cells to generate tumoroids, wherein the cell-compatible support is a sol-gel with a sol-gel transition temperature in the range of 0 to 35° C.,
- d) providing a plurality of test combinations, wherein each test combination comprises a platinum compound,
- e) incubating individual random selections of said tumoroids with each of the test combinations under conditions supporting 3 dimensional maintenance and/or growth of human or other mammalian cells, and
- f) determining whether growth of said tumoroids is inhibited by incubation with said test combinations,
- wherein inhibition of growth of said tumoroids by a given test combination is indicative of efficacy of treatment of colon cancer in said individual with the platinum compound of said test combination.
Dependencies are inferred from the claim text.