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US 12,761,208 B2Granted
IPSC-derived astrocytes and methods of use thereof
AssigneesFUJIFILM Cellular Dynamics, Inc., FUJIFILM Holdings America Corporation
InventorsDeepika Rajesh, Christie Savic, Sarah Burton, Ali Fathi, Rachel Lewis, Robert Bradley, Jing Liu
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Abstract
The present disclosure provides method for producing neural precursor cells with a glial bias from induced pluripotent cells and further differentiating the neural precursor cells to astrocytes. Further provided herein are methods for the use of the astrocytes for screening assays, models mimicking the human brain, and cell therapy.
Claims21
An in vitro method for producing astrocytes from induced pluripotent stem cells (iPSCs) comprising:
- (a) obtaining a starting population of neural precursor cells (NPCs) derived from iPSCs;
- (b) culturing the NPCs in the presence of leukemia inhibitory factor protein (LIF), Ciliary-Derived Neurotrophic Factor protein (CNTF), oncostatin-M protein (OSM), Jagged 1 Fc chimera protein (JAGG1), and EGF for a period of time sufficient to produce astrocyte progenitor cells (APCs);
- and
- (c) further culturing the APCs in the presence of LIF, CNTF and a lipid concentrate comprising arachidonic acid, cholesterol, DL-alpha-Tocopherol Acetate, linoleic acid, linolenic acid, myristic acid, oleic acid, palmitic acid, palmitoleic acid, and/or stearic acid for a period of time sufficient to produce a population of astrocytes.
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Dependencies are inferred from the claim text.