Integrating sphere-based fluorescence detection system for real-time quantitative PCR device

An integrating sphere-based fluorescence detection system for real-time quantitative PCR device, comprising:

• a. an upright structure having a top portion, a central portion and a bottom portion;
• b. a PCR sample plate placed on the bottom portion and configured to hold a plurality of sample wells;
• c. an integrating sphere disposed in the central portion and configured to receive an excitation light and diffusely distribute the excitation light within the integrating sphere, the integrating sphere has a top side, a bottom side, and an opening;
• d. a light source mounted on one side of the upright structure and configured to generate the excitation light and introduce the excitation light into the integrating sphere through the opening;
• e. a dichroic mirror mounted on the top side of the integrating sphere and positioned in front of the light source, wherein the dichroic mirror is configured to reflect the excitation light from the light source into the integrating sphere;
• f. a camera positioned on the top portion and configured to capture fluorescence emission light emitted from the plurality of sample wells in response to the excitation light generated by the light source;
• wherein, the excitation light generated by the light source is projected onto the PCR sample plate which contains multiple liquid samples and the excitation light entering the integrating sphere is uniformly distributed on an inner surface of the integrating sphere after multiple scatterings and the corresponding fluorescence emission light travels upward through the dichroic mirror and is comparison of fluorescence signals from the plurality of sample wells, wherein the fluorescence emission light is measured as an indication of detected DNA quantity.