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AssigneeCornell University
InventorRomulo Hurtado
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A method for rendering biological tissue sufficiently optically transparent for three-dimensional light microscopy imaging, comprising incubating biological tissue with an optical clearing solution, wherein the optical clearing solution comprises: (i) 20-50 wt % formamide, (ii) 10-90 wt % glycerol, and (iii) water as remainder. Also described herein is a method for ridding tissue of blood to make them amenable for optical clearing, comprising incubating biological tissue in a decolorizing solution, wherein the decolorizing solution comprises: (i) 0.5-3 wt % hydrogen peroxide, (ii) 0.05-1 wt % sodium azide, (iii) 5-20 wt % DMSO, and (iv) phosphate buffered saline as a remained. Also described herein is a method for reducing auto-fluorescence in biological tissue to permit imaging of the biological tissue in a fluorescence-based imaging technique with enhanced resolution, wherein the auto-fluorescence quenching solution comprises: 1-100 mM ammonium bicarbonate, (ii) 20-500 μM copper sulfate, (iii) 5-20 wt % DMSO, and (iv) water as remainder.
A method for rendering biological tissue sufficiently optically transparent for three-dimensional light microscopy imaging, the method comprising incubating biological tissue with an optical clearing solution for sufficient time for the optical clearing solution to impregnate the biological tissue and make the biological tissue sufficiently optically transparent for three-dimensional light microscopy imaging, wherein said biological tissue is impregnated with the optical clearing solution by impregnating the biological solution in successive steps comprising:
A method for rendering biological tissue sufficiently optically transparent for three-dimensional light microscopy imaging, the method comprising incubating biological tissue with an optical clearing solution for sufficient time for the optical clearing solution to impregnate the biological tissue and make the biological tissue sufficiently optically transparent for three-dimensional light microscopy imaging, wherein said optical clearing solution comprises (i) formamide in an amount of 20-50 wt %, (ii) glycerol in an amount of 10-90 wt %, and (iii) water as remainder;
A method for rendering biological tissue sufficiently optically transparent for three-dimensional light microscopy imaging, the method comprising incubating biological tissue with an optical clearing solution for sufficient time for the optical clearing solution to impregnate the biological tissue and make the biological tissue sufficiently optically transparent for three-dimensional light microscopy imaging, wherein said optical clearing solution comprises (i) formamide in an amount of 20-50 wt %, (ii) glycerol in an amount of 10-90 wt %, and (iii) water as remainder;
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