Kits and assays for determining bacterial cell viability
AssigneeJosef D. Franke
InventorJosef D Franke
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Abstract
Provided herein are kits comprising (a) erythrosin B (EB); (b) adsorbent; and (c) instructions for use. Also provided are methods of determining the percentage of dead bacteria in a bacterial cell population. The methods comprise (a) obtaining a bacterial cell population; (b) contacting the bacterial cell population with an erythrosin B (EB) solution; (c) contacting the bacterial cell population and EB with an adsorbent to remove excess EB; (d) transferring the non-adsorbed bacterial cell population and EB solution; and (e) determining the percentage of dead bacteria in the bacterial cell population.
Claims23
A kit for determining bacterial cell viability in a bacterial cell population, the kit comprising:
- (a) erythrosin B (EB);
- (b) an adsorbent capable of selectively binding EB and not bacterial cells;
- and
- (c) instructions for use,
- wherein the erythrosin B (EB) and adsorbent are used for determining bacterial cell viability in a bacterial cell population.
Dependencies are inferred from the claim text.