Relevant products and use of inorganic pyrophosphatase in synthesis of nucleotide strands
AssigneeHongene Biotech Pte. Ltd.
InventorsMinzhi Wei, Min Guo
Life of this patent
Estimating the term…
Abstract
The present disclosure discloses relevant products and use of inorganic pyrophosphatase in synthesis of nucleotide strands, relating to the technical field of nucleic acid synthesis. In the present disclosure, by adding the inorganic pyrophosphatase in a synthesis process of nucleotide strands, byproducts produced during ligation reaction can be greatly reduced or eliminated, a reaction rate is accelerated, and further a conversion rate and purity of a target product are improved, thereby being beneficial to fully exerting the efficacy of the nucleotide strands of interest, and providing a new approach for large-scale synthesis of high-quality nucleotide strands.
Claims4
A method for synthesizing a nucleotide strand, comprising:
- performing synthesis using an enzymatic ligation reagent, wherein the enzymatic ligation reagent comprises inorganic pyrophosphatase and nucleic acid ligase, wherein the inorganic pyrophosphatase and the nucleic acid ligase coexist in a reaction solution;
- the nucleic acid ligase comprises a double-stranded ligase;
- a substrate for the synthesis of the nucleotide strand is a nicked double-stranded nucleic acid molecule and/or a nucleic acid substrate capable of forming the nicked double-stranded nucleic acid molecule;
- and
- optionally, conditions of the synthesis comprise:
- a temperature of 15-55° C. and a duration of 1-48 h.
Dependencies are inferred from the claim text.