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US 12,760,337 B2Granted
Succinic acid production using Issatchenkia
AssigneeThe Board of Trustees of the University of Illinois
InventorsHuimin Zhao, Vinh Tran
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Abstract
Provided herein are genetically modified Issatchenkia yeast and fermentation methods for producing succinic acid.
Claims22
A recombinant Issatchenkia yeast comprising:
- (a) heterologous polynucleotides encoding pyruvate carboxylase (PYC), malate dehydrogenase (MDH), fumarase (FUMR) and fumarate reductase (FDR);
- (b) a heterologous polynucleotide encoding dicarboxylic acid transporter (MAE1);
- (c) a deleted or non-functional pyruvate decarboxylase (PDC) gene;
- (d) a deleted or non-functional glycerol 3-phosphate dehydrogenase (GPD) gene;
- and
- (e) a heterologous polynucleotide encoding glycerol dehydrogenase (GDH).
3 more elements
A recombinant Issatchenkia yeast comprising:
- (a) heterologous polynucleotides encoding pyruvate carboxylase (PYC), malate dehydrogenase (MDH), fumarase (FUMR) and fumarate reductase (FDR);
- (b) a heterologous polynucleotide encoding dicarboxylic acid transporter (MAE1);
- (c) a deleted or non-functional pyruvate decarboxylase (PCD) gene;
- (d) a deleted or non-functional glycerol 3-phosphate dehydrogenase (GPD) gene;
- (e) a deleted or non-functional JEN carboxylate transporter gene;
- (f) a heterologous polynucleotide encoding glycerol dehydrogenase (GDH);
- (g) an overexpressed dihydroxyacetone kinase (DAK) gene;
- and
- (h) a deleted or non-functional g3837 hexokinase or a heterologous polynucleotide encoding invertase (SUC2).
6 more elements
Dependencies are inferred from the claim text.