Biosynthesis of cannabinoid precursors using novel aromatic prenyl transferases
AssigneeNational University of Singapore
InventorsMaybelle Darlene Kho Go, Kevin Jie Han Lim, Yan Ping Lim, Wen Shan Yew
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Abstract
A method for producing a cannabinoid precursor by contacting a substrate and geranyl pyrophosphate or farnesyl pyrophosphate with an NphB orthologue. The NphB orthologue is from an organism other than Cannabis sativa, and the substrate can be 2,4-dihydroxy-6-pentylbenzoic acid or 2,4-dihydroxy-6-propylbenzoic acid. Also disclosed is a recombinant cell of Yarrowia lipolytica, carrying in its genome a nucleic acid encoding an NphB orthologue from an organism other than Cannabis sativa such that the NphB orthologue is expressed in the recombinant cell.
Claims8
A method for producing a cannabinoid precursor, the method comprising contacting 2,4-dihydroxy-6-pentylbenzoic acid (olivetolic acid) and geranyl pyrophosphate with an NphB orthologue comprising the amino acid sequence of any one of SEQ ID NOs:
- 1-8 to produce cannabigerolic acid (CBGA) and at least one other product having a same molecular formula as CBGA and a longer retention time than CBGA as determined by liquid chromatography mass spectrometryl.
Dependencies are inferred from the claim text.