Bioactive glass as nucleic acid carriers with pH switch control-releasing
AssigneeCorning Incorporated
InventorsPei-Chen Chiang, Qiang Fu, Cheng-I Hsu, Wei-Lun Hsu
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Abstract
A pH-switchable carrier composition includes a plurality of bioactive glass particles, wherein each of the bioactive glass particle is optionally at least a partially coated with a surface modifier; wherein the bioactive glass particles, with or without, the surface modifier can bind to a nucleic acid compound upon contact at pH in the range of about 7 to about 11, and exhibit controlled release of the nucleic acid compound at pH in the range of about 5 to 6.
Claims32
A pH-switchable carrier composition comprising:
- a plurality of bioactive glass particles, wherein each of the bioactive glass particles is optionally at least partially coated with a surface modifier;
- wherein the plurality of bioactive glass particles comprise a nucleic acid compound non-covalently bound to a surface of the bioactive glass particles;
- and
- wherein the bioactive glass particles, with or without the surface modifier, are configured to bind to the nucleic acid compound upon contact at pH in the range of 7 to 11, and exhibit controlled release of the nucleic acid compound at pH in the range of 5 to 6.
A plurality of bioactive glass particles, comprising:
- optionally a surface modifier at least partially coating the plurality of bioactive glass particles;
- a nucleic acid compound non-covalently bound to a surface of the bioactive glass particles;
- and
- wherein the bioactive glass particles, with or without the surface modifier, are configured to bind to the nucleic acid compound upon contact at pH in the range of 7 to 11, and exhibit controlled release of the nucleic acid compound at pH in the range of 5 to 6;
- and
- wherein the bioactive glass particle mainly comprises, expressed in terms of weight percent on the oxide basis:
- (a) from about 30% to about 75% SiO2, from about 2% to about 40% CaO and from about 3% to about 15% P2O5;
- or
- (b) from about 30% to about 70% B2O3, from about 5% to about 25% CaO and from about 1% to about 10% P2O5.
A method of manufacturing a pH-switchable carrier composition, the method comprising:
- processing a bioactive glass into fine particles;
- mixing the fine particles of bioactive glass with a liquid medium to form a solution;
- optionally modifying the surface of the bioactive glass particles in the solution with a surface modifier to form surface-modified bioactive glass particles,
- contacting the bioactive glass particles or the surface-modified bioactive glass particles with a nucleic acid compound in a liquid medium to form an admixture;
- and
- incubating the admixture under conditions in which the nucleic acid compound non-covalently binds to the bioactive glass particles or the surface-modified bioactive glass particles,
- wherein the bioactive glass particles, with or without the surface modifier, are configured to bind to the nucleic acid compound upon contact at pH in the range of 7 to 11, and exhibit controlled release of the nucleic acid compound at pH in the range of 5 to 6.
Dependencies are inferred from the claim text.