Genetic means to increase neutral oil in vegetative tissues of plants by conditional induction of membrane lipid hydrolysis
AssigneeThe Curators of the University of Missouri
InventorsJeong-Kyu Abraham Koo, Athen N. Kimberlin
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Abstract
The disclosure relates to compositions and methods for increasing oil content of the vegetative tissues of plants. The compositions comprise polynucleotides encoding phospholipase A1 (PLA1) polypeptides and plants, plant parts, plant cells, and seeds comprising such polynucleotides. The methods for increasing oil content of the vegetative tissues of plants comprise introducing a polynucleotide encoding PLA1 polypeptides into the plant. Expression of these PLA1 polynucleotides result in a large accumulation of triacyl glycerides or neutral lipids in vegetative tissues such as leaves that normally contain very little amount of such oil.
Claims19
A plant, or a part thereof, that inducibly produces triacylglycerols in a vegetative tissue, comprising:
- a polynucleotide encoding a phospholipase A1 (PLA1) polypeptide operatively linked to a heterologous chemical-inducible promoter functional in a plant cell, wherein the PLA1 polypeptide has at least 95% sequence identity to SEQ ID NO:
- 1, and wherein the plant produces triacylglycerols in the vegetative tissue of the plant when contacted with an exogenous chemical inducer of the promoter.
Dependencies are inferred from the claim text.