Anti-CD3 aptamers for use in cell targeting and labeling
OwnerAnna Miodek, Frédéric Mourlane +2inventors
InventorsAnna Miodek, Frédéric Mourlane, Cécile Bauche, Renaud Vaillant
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Abstract
High affinity aptamer sequences recognizing CD3 protein complex on cell surfaces are provided. The aptamers can be used as targeting moieties for delivery vehicles or as molecular components for immunotherapy, immunodiagnostics, or for isolating, purifying, or characterizing CD3+ T cells in a subject.
Claims16
A vehicle for delivering an agent, a dye, a functional group for covalent coupling or a biologically active agent to T cells, wherein the vehicle comprises (i) an aptamer comprising the sequence GX1TTX2GX3X4X5X6CX7GGX8CTGGX9G, wherein X1 is A or G;
- X2 is T or G;
- X3 and X7, X9 are G or C;
- X4 is T or C;
- X5 is A or T;
- X6 is T, C, or G;
- X8 is A or C (SEQ ID NO:111) or a variant thereof having one or more of said bases substituted with a non-naturally occurring base or one or more of said bases omitted and wherein the aptamer binds to CD3 ε/γ or CD3 ε/δ, and (ii) said agent, dye, functional group for covalent coupling, or biologically active agent.
A method of isolating T cells from a subject, the method comprising using an aptamer to isolate T cells from the subject, wherein the aptamer comprises the sequence GX1TTX2GX3X4X5X6CX7GGX8CTGGX9G, wherein X1 is A or G;
- X2 is T or G;
- X3 and X7, X9 are G or C;
- X4 is T or C;
- X5 is A or T;
- X6 is T, C, or G;
- X8 is A or C (SEQ ID NO:
- 111) or a variant thereof having one or more of said bases substituted with a non-naturally occurring base or one or more of said bases omitted or the corresponding nucleotide replaced with a linker, and wherein the aptamer binds to CD3 ε/γ or CD3 ε/δ.
Dependencies are inferred from the claim text.