Vector set for measuring transposase activity, kit, transposase activity measuring method, and cell separation method
AssigneeKabushiki Kaisha Toshiba
InventorsEmi Nozaki, Eiichi Akahoshi, Mitsuko Ishihara
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Abstract
According to one embodiment, a vector set includes a first vector and a second vector. The first vector includes a transposase target sequence, a first promoter sequence ligated to downstream of the transposase target sequence, and a first reporter gene ligated to downstream of the first promoter sequence. The second vector includes a 5′-side transposase recognition sequence, a 3′-side transposase recognition sequence, and a first enhancer sequence arranged therebetween.
Claims17
A vector set comprising a first vector comprising a transposase target sequence, a first promoter sequence ligated downstream of the transposase target sequence, and a first reporter gene ligated downstream of the first promoter sequence;
- and
- a second vector comprising a second vector comprising, in order from its 5′ end to its 3′ end, a second promoter sequence, a 5′-side fragment of a second reporter gene, 5′-side of a transposase recognition sequence, a first enhancer sequence, 3′-side of the transposase recognition sequence, and a 3′-side fragment of the second reporter gene;
- and wherein the 5′-side fragment of the second reporter gene and the 3′-side fragment of the second reporter gene are obtained by dividing the second reporter gene into the 5′-side fragment and the 3′-side fragment.
A vector set comprising:
- a first vector including a sequence of a gene expression unit that comprises, in order from its 5′ end to its 3′ end, 5′ side of a first enhancer sequence, a transposase target sequence, 3′ side of the first enhancer sequence, a first promoter sequence or in order from its 5′ end to its 3′ end, the first enhancer sequence, 5′ side of the first promoter sequence, the transposase target sequence, 3′ side of the first promoter sequence, and the first reporter gene;
- and
- a second vector, in order from its 5′ end to its 3′ end, including a 5′-side of a transposase recognition sequence, a sequence involved in expression of the first reporter gene, a 3′-side of the transposase recognition sequence, and a second promoter sequence.
Dependencies are inferred from the claim text.