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US 12,760,294 B2Granted
Mutant aminoacyl-tRNA synthetases
AssigneeB. G. Negev Technologies and Applications Ltd., At Ben-Gurion University
InventorsMiriam Amiram, Sigal Gelkop, Bar Israeli, Daniela Strugach
Life of this patent
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Abstract
Mutant aminoacyl-tRNA synthetase (aaRS) proteins are provided. Nucleic acid molecules encoding the mutant aaRSs, orthogonal translation systems comprising the mutant aaRSs or nucleic acid molecules, cells comprising the orthogonal translation systems, as well as methods of using same are also provided.
Claims18
A mutant aminoacyl-tRNA synthetase (aaRS) comprising an amino acid sequence of an aaRS with at least 85% sequence identity to SEQ ID NO:
- 1 and comprising:
- a) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- and leucine 162 mutated to serine or leucine 162 mutated to arginine;
- b) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- and alanine 167 mutated to phenylalanine;
- c) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine and tyrosine 32 mutated to leucine or tyrosine 32 mutated to glycine;
- d) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- alanine 167 mutated to phenylalanine;
- and tyrosine 32 mutated to leucine or tyrosine 32 mutated to glycine;
- e) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- and leucine 65 mutated to valine or leucine 65 mutated to glycine;
- f) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- alanine 167 mutated to phenylalanine;
- and leucine 65 mutated to valine or leucine 65 mutated to glycine;
- g) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- tyrosine 32 mutated to leucine or tyrosine 32 mutated to glycine;
- and leucine 65 mutated to valine or leucine 65 mutated to glycine;
- or
- h) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- alanine 167 mutated to phenylalanine;
- tyrosine 32 mutated to leucine or tyrosine 32 mutated to glycine;
- and leucine 65 mutated to valine or leucine 65 mutated to glycine.
34 more elements
A variant of a protein comprising SEQ ID NO:
- 1, wherein the variant comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 1 comprising
- a) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- and leucine 162 mutated to serine or leucine 162 mutated to arginine;
- b) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- and alanine 167 mutated to phenylalanine;
- c) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine and tyrosine 32 mutated to leucine or tyrosine 32 mutated to glycine;
- d) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- alanine 167 mutated to phenylalanine;
- and tyrosine 32 mutated to leucine or tyrosine 32 mutated to glycine;
- e) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- and leucine 65 mutated to valine or leucine 65 mutated to glycine;
- f) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- alanine 167 mutated to phenylalanine;
- and leucine 65 mutated to valine or leucine 65 mutated to glycine;
- g) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- tyrosine 32 mutated to leucine or tyrosine 32 mutated to glycine;
- and leucine 65 mutated to valine or leucine 65 mutated to glycine;
- or
- h) aspartic acid 158 mutated to glycine;
- isoleucine 159 mutated to tyrosine;
- leucine 162 mutated to serine or leucine 162 mutated to arginine;
- alanine 167 mutated to phenylalanine;
- tyrosine 32 mutated to leucine or tyrosine 32 mutated to glycine;
- and leucine 65 mutated to valine or leucine 65 mutated to glycine.
35 more elements
Dependencies are inferred from the claim text.