# US 12,760,293 B2 · High efficiency oxalate-degrading enzymes for degradation of insoluble and soluble oxalate

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- Number: US 12,760,293 B2
- Document ID: US12760293B2
- Status: Granted
- Kind: B2
- Application number: 17/708,752
- Filed: 2022-03-30
- Published: 2022-09-22
- Granted: 2026-10-13
- Earliest claimed: 2015-04-02
- Assignees: Orenzymes LLC
- Applicants: Orenzymes LLC
- Inventors: Aaron B. Cowley; Helena Cowley; Qin Yan; Qingshan Li
- Web page: https://patentgrep.com/patent/US12760293B2

## CPC

- C12N 9/88: Lyases (4.)
- C12Y 401/01002: Oxalate decarboxylase (4.1.1.2)
- C12Y 401/01008: Oxalyl-CoA decarboxylase (4.1.1.8)
- A61K 38/44: Oxidoreductases (1)
- A61K 38/51: Lyases (4)
- C12Y 102/03004: Oxalate oxidase (1.2.3.4)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Disclosed herein are oxalate inducing enzymes with pH and thermal stability and methods of using for treatment of oxalate related conditions for in food processing.

## Claims

### Claim 1

A method of reducing oxalate in a mammal, the method comprising administering an oxalate-reducing effective amount of a composition to the mammal, wherein the composition comprises one or more oxalate-degrading enzymes comprising the amino acid sequence of any one of SEQ ID NOs: 3-39.

### Claim 2

A method reducing oxalate in a mammal, the method comprising administering an oxalate-reducing effective amount of a composition to the mammal, wherein the composition comprises one or more oxalate-degrading enzymes that comprise the amino acid sequence of any one of SEQ ID Nos: 3-39, or an amino acid sequence at least 95% identical thereto with at least one of the following amino acids at positions corresponding to SEQ ID NO: 2: 97-H, 98-W, 99-H, 103-E, 143-H, 280-H, 281-W, 282-H, 286-E or 326-H.