Method for targeted modification of a plant genome sequence

A method of producing a genetically modified plant, comprising:

• providing a plant genome editing system for targeted modification of a plant genome comprising:
• i) a fusion protein and/or an expression construct comprising a nucleotide sequence encoding the fusion protein, wherein the fusion protein comprises a CRISPR nickase and a reverse transcriptase;
• and
• ii) at least a pair of pegRNAs and/or an expression construct comprising a nucleotide sequence encoding the at least a pair of pegRNAs,
• wherein each of the pegRNAs comprises, in the direction from 5′ to 3′, a guide sequence, a scaffold sequence, a reverse transcription (RT) template sequence and a primer binding site (PBS) sequence,
• wherein each of the pegRNAs can form a complex with the fusion protein and target the fusion protein to a target sequence in the genome, so that a nick is formed in the target sequence;
• and
• introducing the plant genome editing system into at least one plant, thereby resulting in a modification in the genome of the at least one plant,
• wherein two pegRNAs in the pair of pegRNAs are configured to target different target sequences on the same strand of the genomic DNA, or the two pegRNAs in the pair of pegRNAs are configured to target the target sequences on different strands of the genomic DNA,
• wherein nicks induced by the two pegRNAs are located on two sides of the site to be modified, respectively.