Early mesenchymal stem cells with reduced aging and preserved stem cell ability, and culturing method therefor
AssigneesEncell Co., Ltd., Samsung Life Public Welfare Foundation
InventorsHong Bae Jeon, Sang Eon Park, Jong Wook Chang
Life of this patent
Estimating the term…
Abstract
A method for culturing mesenchymal stem cells is disclosed. The method includes steps of: primary culturing mesenchymal stem cells in a medium containing umbilical cord blood serum; and subculturing the mesenchymal stem cells in a medium containing fetal bovine serum. The method for culturing mesenchymal stem cells may maintain stemness by reducing the differentiation and aging of stem cells, and in particular, may achieve a remarkably high proliferation rate.
Claims7
A method for culturing mesenchymal stem cells, comprising the steps of:
- primary culturing isolated human mesenchymal stem cells derived from an umbilical cord in a medium containing umbilical cord blood serum;
- and
- subculturing the isolated human mesenchymal stem cells derived from the umbilical cord in a medium containing fetal bovine serum.
A method for reducing aging of mesenchymal stem cells, comprising the steps of:
- isolating mesenchymal stem cells from isolated tissue using a mixed enzyme solution of collagenase and hyaluronidase, wherein the isolated tissue is an umbilical cord;
- primary culturing the isolated mesenchymal stem cells in a medium containing umbilical cord blood serum;
- and subculturing the mesenchymal stem cells in a medium containing fetal bovine serum.
Dependencies are inferred from the claim text.