Expansion of hematopoietic stem cells
AssigneeMesoblast International Sarl
InventorsSilviu Itescu, Paul Simmons
Life of this patent
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Abstract
The present disclosure relates to methods and compositions for expansion of human hematopoietic stem cells. The present disclosure also relates to methods of treatment involving the use of the expanded HSCs.
Claims20
A method of expanding hematopoietic stem cells, comprising culturing a population of hematopoietic cells in the presence of mesenchymal lineage precursor or stem cells (MLPSCs) which express TNAP or undifferentiated progeny thereof and at least one histone deacetylase inhibitor (HDACi) such that hematopoietic stem cells having the phenotype CD34+ are expanded when compared to the population of hematopoietic cells prior to culturing in the presence of mesenchymal lineage precursor or stem cells (MLPSCs) and at least one histone deacetylase inhibitor (HDACi).
Dependencies are inferred from the claim text.