Nonviral modification of T cell gene expression
AssigneeGlobal Life Sciences Solutions Canada ULC
InventorsAnitha Thomas, Andrew William Brown, Rebecca Anne Grace De Souza, Tara Fernandez
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Abstract
There is provided a lipid mix composition comprising ionizable lipid, a structural lipid such as DSPC, a sterol, and a surfactant such as polysorbate 80, polyoxyethylene (10) stearyl ether, polyoxyethylene (20) stearyl ether, or D-α-Tocopherol polyethylene glycol 1000 succinate. The lipid mix compositions find particular use in transfecting difficult to transfect cells and maintaining the viability of those cells. The lipid mix compositions are particularly well suited to T cell transfection ex vivo.
Claims11
A lipid mix composition for forming lipid particles in association with a nucleic acid, for use in transfecting the nucleic acid into T cells, the composition comprising:
- about 40-50 Mol % ionizable lipid,
- about 10-20 Mol % a structural lipid, wherein the structural lipid comprises distearoylphosphatidylcholine (DSPC),
- about 35 to 40 Mol % sterol, and
- about 0.1-3 Mol % stabilizer, wherein the stabilizer comprises polyoxyethylene (10) stearyl ether, D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS), or combinations thereof.
Dependencies are inferred from the claim text.