Compositions and methods for producing placental cells
AssigneeRegents of the University of Minnesota
InventorsSamira Azarin, Casim A. Sarkar, Kristen A. Lemke
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Abstract
Certain embodiments provide a method of producing a population of induced multipotent placental cells, the method comprising culturing a population of pluripotent stem cells in the presence of an induction media comprising a retinoid, and optionally a Wnt signaling agonist. Certain embodiments also provide cells, compositions, kits and methods of use thereof.
Claims13
A method of producing a population of induced multipotent placental cells, the method comprising culturing a population of pluripotent stem cells in the presence of an induction media comprising a retinoid and a Wnt signaling agonist for between 4 to 6 days, under conditions suitable to produce the population of induced multipotent placental cells, wherein the induction media comprises from 0.1 to 10 μM of the retinoid and from 2 to 20 μM of the Wnt signaling agonist;
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- wherein the retinoid is retinoic acid, or a salt thereof, and the Wnt signaling agonist is selected from the group consisting of BIO, SB216763, CHIR-99021, and salts thereof.
Dependencies are inferred from the claim text.