Chimeric antigen receptor modified T-cells (CAR-T) for the treatment of hematological and solid tumor cancers
AssigneeThe Regents of the University of California
InventorsCharles E. Prussak, Thomas J. Kipps, George F. Widhopf, II, Christopher S. Oh
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Abstract
Provided herein is a chimeric antigen receptor (CAR) and CAR-expressing immune cells that target human RORI expressed aberrantly on a tumor cancers. Described herein are chimeric antigen receptors that target human ROR-1, cell compositions expressing the chimeric antigen receptors, and methods and uses of the chimeric antigen receptors and/or the cell compositions. The chimeric antigen receptors described herein can be expressed by the T lymphocytes isolated from an individual afflicted with cancer and re-administered to the individual.
Claims21
A chimeric antigen receptor comprising:
- i. an antigen binding region, wherein the antigen binding region specifically binds ROR-1 and wherein the antigen binding region comprises a light chain variable domain and a heavy chain variable domain;
- (a) wherein the light chain variable domain comprises a CDR L1 as set forth in SEQ ID NO:43, a CDR L2 as set forth in SEQ ID NO:44 and a CDR L3 as set forth in SEQ ID NO:45;
- and the heavy chain variable domain comprises a CDR H1 as set forth in SEQ ID NO:46, a CDR H2 as set forth in SEQ ID NO:47, and a CDR H3 as set forth in SEQ ID NO:48;
- or
- (b) wherein the light chain variable domain comprises a CDR L1 as set forth in SEQ ID NO:49, a CDR L2 as set forth in SEQ ID NO:50 and a CDR L3 as set forth in SEQ ID NO:51;
- and the heavy chain variable domain comprising a CDR H1 as set forth in SEQ ID NO:52, a CDR H2 as set forth in SEQ ID NO:53, and a CDR H3 as set forth in SEQ ID NO:
- 54;
- ii. a spacer domain, wherein the spacer domain comprises an amino acid sequence of SEQ ID NO:29 or SEQ ID NO:
- 41;
- iii. a transmembrane domain;
- and
- iv. an intracellular domain
- wherein the chimeric antigen receptor binds to a cell expressing ROR-1.
Dependencies are inferred from the claim text.