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US 12,760,092 B2Granted
Glycosylated single chain immunoglobulin domains
AssigneesVib Vzw, Universiteit Gent
InventorsNico Callewaert, Loes Van Schie, Wander Van Breedam
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Abstract
The present application relates to glycosylated immunoglobulin domains. The invention provides nucleotide sequences encoding polypeptides comprising immunoglobulin variable domains with engineered glycosylation acceptor sites. Accordingly, the invention provides immunoglobulin variable domain proteins modified with selected glycans and specific glycan-conjugates thereof. Also provided herein are methods for the production of glycosylated immunoglobulin variable domains and glycan-conjugates thereof.
Claims9
A polynucleotide encoding a polypeptide, wherein the encoded polypeptide comprises:
- an immunoglobulin single variable domain (ISVD) derived from a camelid heavy chain antibody and having the same antigen specificity as the camelid heavy chain antibody from which it is derived,
- wherein the ISVD comprises an amino acid sequence that comprises 4 framework regions (FR) and 3 complementarity determining regions (CDR) according to the following formula (1):
- FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 (1), and
- wherein the ISVD contains an NXT, NXS, NXC or NXV motif, in which X can be any amino acid, such that the asparagine residue of the NXT/NXS/NXC/NXV motif is present at an amino acid selected from positions 50 and/or 52 of the ISVD according to the AHo numbering convention.
1 more element
Dependencies are inferred from the claim text.