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US 12,758,970 B2Granted
Configurable substrate of a fluidic device
AssigneeXbiologix, Inc.
InventorsRyan A. Mehl, Eduardo Ceballos, Kevin F. Jones
Life of this patent
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Abstract
A method of forming a configurable substrate includes depositing a volume of trans-cyclooctene on a configurable substrate of a fluidic device, and forming a bioorthogonal tethered protein on the configurable substrate. The bioorthogonal tethered protein may be formed by attaching a tetrazine-modified protein or tetrazine-modified functional protein fragment to the trans-cyclooctene, wherein the tetrazine-modified protein or tetrazine-modified functional protein fragment is configured to bind to a target analyte.
Claims20
A method, comprising:
- forming a plurality of bioorthogonal tethered proteins on a configurable substrate by attaching a tetrazine-modified protein or a tetrazine-modified functional protein fragment to trans-cyclooctene for each of the plurality of bioorthogonal tethered proteins, wherein the plurality of bioorthogonal tethered proteins includes a first ligand and a second ligand, the first ligand and second ligand being different from one another or being formed in a chain, and wherein a concentration and an orientation of the plurality of bioorthogonal tethered proteins are configurable on the configurable substrate.
A configurable substrate of a fluidic device, comprising:
- an assay region of the configurable substrate including a plurality of bioorthogonal tethered proteins, wherein the plurality of bioorthogonal tethered proteins includes:
- a plurality of trans-cyclooctenes tethered to a surface of the configurable substrate;
- and
- a plurality of tetrazine-modified proteins or tetrazine-modified functional protein fragments tethered to the plurality of trans-cyclooctenes, wherein the plurality of tetrazine-modified proteins or tetrazine-modified functional protein fragments include a first ligand and a second ligand, the first ligand and second ligand being different from one another or being formed in a chain, wherein a concentration and an orientation of the plurality of bioorthogonal tethered proteins are configured on the configurable substrate.
1 more element
Dependencies are inferred from the claim text.