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US 12,758,967 B2Granted
Products and methods to isolate mitochondria
AssigneesChildren's Medical Center Corporation, Beth Israel Deaconess Medical Center, Inc.
InventorsJames D. McCully, Douglas B. Cowan, Christina A. Pacak, Sidney Levitsky
Life of this patent
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Abstract
Filtration apparatuses, kits, and methods for rapid isolation of intact, viable mitochondria from tissues are described with mitochondria isolated by differential filtration through nylon mesh filters. Mitochondria can be isolated in less than 30 minutes using the filtration apparatuses, kits, and methods described.
Claims7
A method for isolating a viable mitochondrion, the method comprising:
- providing a cell homogenate comprising a viable mitochondrion;
- passing the cell homogenate through a first filter having a pore-size of about 30 μm to about 50 μm;
- and
- subsequently passing the cell homogenate through a second filter having a pore-size of about 5 μm to about 20 μm, thereby isolating the viable mitochondrion,
- wherein the viable mitochondrion is isolated by passing the cell homogenate through the first and the second filters.
2 more elements
A method for isolating a viable mitochondrion, the method comprising:
- providing a cell homogenate comprising a viable mitochondrion;
- passing the cell homogenate through an apparatus comprising:
- a tubular body comprising a lumen;
- a first filter disposed and secured within the lumen, wherein the filter has a pore-size of about 30 μm to about 50 μm;
- and
- a second filter disposed and secured within the lumen adjacent to the first filter and having a pore-size of about 5 μm to about 20 μm, thereby isolating the viable mitochondrion,
- wherein the viable mitochondrion is isolated by passing the cell homogenate through the first and the second filters.
4 more elements
Dependencies are inferred from the claim text.