# US 12,758,964 B2 · Pipette tip with two positive angle steps > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,758,964 B2 - Document ID: US12758964B2 - Status: Granted - Kind: B2 - Application number: 17/839,258 - Filed: 2022-06-13 - Published: 2023-03-02 - Granted: 2026-10-13 - Earliest claimed: 2021-08-30 - Assignees: Tecan Trading AG - Applicants: Tecan Trading AG - Inventors: Michael Keller - Web page: https://patentgrep.com/patent/US12758964B2 ## CPC - B01L 3/0275: {Interchangeable or disposable dispensing tips} - B01L 3/0293: {for liquids} - B01L 3/5635: {connecting two containers face to face, e.g. comprising a filter} - C12M 33/04: {by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02)} - B01L 2200/025: Align devices or objects to ensure defined positions relative to each other - B01L 2200/026: Fluid interfacing between devices or objects, e.g. connectors, inlet details - B01L 2200/0615: Loss of fluid by dripping - B01L 2200/0689: Sealing - B01L 2300/0858: Side walls - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract A pipette includes an end section, a first section, a second section and a third section aligned along a central axis of the pipette tip. The end section comprises an orifice for aspirating and dispensing a fluid. A first kink is formed in an inner surface of the pipette tip between the first section and the second section, such that an angle between the inner surface of the first section and the central axis is smaller than an angle between the inner surface of the second section and the central axis. A second kink is formed in the inner surface of the pipette tip between the second section and the third section, such that the angle between the inner surface of the second section and the central axis is smaller than an angle between the inner surface of the third section and the central axis. ## Claims ### Claim 1 A pipette tip comprising: an end section, a first section, a second section and a third section aligned along a central axis of the pipette tip; wherein the end section comprises an orifice for aspirating and dispensing a fluid, the first section is adjacent to the end section, the second section adjacent to the first section, and the third section adjacent to the second section; wherein a first kink is formed in an inner surface of the pipette tip between the first section and the second section, such that an angle (α1) between the inner surface of the first section and the central axis is smaller than an angle (α2) between the inner surface of the second section and the central axis; wherein a second kink is formed in the inner surface of the pipette tip between the second section and the third section, such that the angle (α2) between the inner surface of the second section and the central axis is smaller than an angle (α3) between the inner surface of the third section and the central axis, wherein the first kink and the second kink are formed, such that when a fluid level passes one of the first kink and the second kink during aspiration or dispensing of the fluid, a border of the surface of the fluid stays or sticks at the first kink and the second kink due to capillary forces, even when a pressure inside the pipette tip changes; wherein an angle between the inner surface of the first section and the inner surface of the second section is larger than 10° and is smaller than 5°, and wherein an angle between the inner surface of the second section and the inner surface of the third section is larger than 10° and is smaller than 5°, wherein a position of the first kink is arranged, such that an inner volume of the pipette tip between the orifice and the first kink is between 0.3 μl and 0.6 μl. ### Claim 2 (depends on 1) The pipette tip of claim 1, wherein the angle (α1) between the inner surface of the first section and the central axis is larger than 2° and smaller than 5°; wherein the angle (α2) between the inner surface of the second section and the central axis is larger than 3° and smaller than 9°; wherein the angle (α3) between the inner surface of the third section and the central axis is larger than 4° and smaller than 13°. ### Claim 3 (depends on 1) The pipette tip of claim 1, wherein a position of the second kink is arranged, such that an inner volume of the pipette tip between the orifice and the second kink is between 0.8 μl and 1.2 μl. ### Claim 4 (depends on 1) The pipette tip of claim 1, wherein a length of the third section along the central axis is arranged, such that an inner volume of the pipette tip between the orifice and the length is at least a nominal volume of the pipette tip. ### Claim 5 (depends on 1) The pipette tip of claim 1, wherein the inner surface of the first section is conical; wherein the inner surface of the second section is conical; wherein the inner surface of the third section is conical. ### Claim 6 (depends on 1) The pipette tip of claim 1, wherein a further kink is formed in the inner surface of the pipette tip between the end section and the first section. ### Claim 7 (depends on 1) The pipette tip of claim 1, wherein an angle between the inner surface of the end section and the inner surface of the first section is larger than 2° and is smaller than 5°. ### Claim 8 (depends on 1) The pipette tip of claim 1, wherein the inner surface of the end section is cylindrical. ### Claim 9 (depends on 1) The pipette tip of claim 1, wherein a common outer surface of the first section, the second section and the third section is conical. ### Claim 10 (depends on 1) The pipette tip of claim 1, wherein the pipette tip comprises a cylindrical section aligned along the central axis of the pipette tip following the third section; wherein the cylindrical section is longer than the pipette tip between the orifice and the cylindrical section. ### Claim 11 (depends on 1) The pipette tip of claim 1, wherein the pipette tip comprises a filter section aligned along the central axis of the pipette tip, the filter section following the third section; wherein the inner surface of the filter section comprises ribs extending radially around the central axis. ### Claim 12 (depends on 1) The pipette tip of claim 1, wherein the pipette tip comprises an attachment section for attaching the pipette tip to a nozzle of a laboratory automation device for changing a pressure inside the pipette tip; wherein the attachment section is aligned along the central axis of the pipette tip following the third section. ### Claim 13 (depends on 12) The pipette tip of claim 12, wherein the attachment section is formed, such that a further pipette tip is partially insertable into the attachment section to at least the third section, for stacking the further pipette tip and the pipette tip.