Device and method for the preparation of platelet rich plasma

Microfluidic device for preparation of platelet rich plasma (PRP) enriched in non-platelet biomolecules, comprising:

• a first platform adapted for evaporating plasma comprising:
• a first layer comprising a first microchannel formed on a first surface of said first layer;
• a second layer comprising a second microchannel formed on a first surface of said second layer;
• and
• a first permeable membrane with a molecular-weight cutoff (MWCO) between 10 Dalton and 20 kDalton, placed between the first and second layer;
• wherein the first surfaces of the first and second layers face each other and are in contact with the first permeable membrane;
• wherein the first permeable membrane covers the first and second microchannels, such that plasma can flow through the first microchannel and fluid can flow through the second microchannel;
• wherein the first and second microchannels are spatially arranged with respect to each other such that molecules that evaporate from the first microchannel and cross the first permeable membrane are received in the second microchannel;
• wherein the first microchannel comprises a first inlet for inputting plasma and a first outlet for outputting plasma, and the second microchannel comprises a second inlet for inputting fluid and a second outlet for outputting fluid;
• a second platform adapted for dialyzing plasma comprising:
• a third layer comprising a third microchannel formed on a first surface of said third layer;
• a fourth layer comprising a fourth microchannel formed on a first surface of said fourth layer;
• and
• a second permeable membrane with a molecular-weight cutoff (MWCO) between 100 Dalton and 1 kDalton, placed between the third and fourth layer;
• wherein the first surfaces of the third and fourth layers face each other and are in contact with the second permeable membrane;
• wherein the second permeable membrane covers the third and fourth microchannels, such that plasma can flow through the third microchannel and fluid can flow through the fourth microchannel;
• wherein the third and fourth microchannels are spatially arranged with respect to each other such that molecules that diffuse from the third microchannel and across the second permeable membrane are received in the fourth microchannel;
• wherein the third microchannel comprises a third inlet for inputting plasma and a third outlet for outputting plasma, and the fourth microchannel comprises a fourth inlet for inputting fluid and a fourth outlet for outputting fluid;
• and
• wherein the first outlet is in fluid communication with the third inlet, or the third outlet is in fluid communication with the first inlet.